We narrowed to 161 results for: jun
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TypeBlog Post... 6.2 (2002): 272. PubMed PMID: 12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns...
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Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...fusions to connexin localized correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...predecessor. Important features may include cloning junctions (where most sequence assembly errors occur), deletions... -
The Future of Research Symposium Boston 2015
TypeBlog Post...Shaping the Future of Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID... -
Plasmids 101: Cre-lox
TypeBlog Post...alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post... vectors Behavioral testing is often done in conjunction with a genetic manipulation so that behavior ... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule FRET for understanding biomolecular... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3... -
Hot Plasmids February 2024
TypeBlog Post...cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem by optimizing the... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...Shaping the Future of Research: a perspective from junior scientists."F1000Research 3 (2014). Pubmed PMID... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...improving my skills and sharing with other more junior leaders what I had experienced and learnt. Opening... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...clones displayed proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...while a catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-K (2018) Global analysis of ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated total ... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...., Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell... -
MXS Chaining
TypeBlog Post...acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810. ... -
Lentiviral Vector Uses and Overview
TypeBlog Post...RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM, Rebello T. J Gene Med. 2013 Feb;15... -
Plasmids 101: Colony PCR
TypeBlog Post... insert orientation, and the sequences of the junctions between the plasmid and insert DNA. Colony PCR...