.... Widening your search The first step towards inviting serendipity into your research lies in expanding...approaches One approach to achieve this goal could involve participating in interdisciplinary seminars, such...Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint areas of my career...openness to unexpected possibilities, you not only invite serendipity into your research but also cultivate...

...various cas genes not only to store a record of invading phages but to also destroy the phage upon re-exposure... the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November 2013. Author...systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution of...research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know.  References...

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...less time and resources resupplying our current inventory and more time providing new materials to the research...

...the superheroes win in the end. But we’re still invested in seeing how they do it.    Finding Conflict...even when we know the ending, we can still get invested in the journey? The same thing applies for science...communication, use conflict to get your audience invested in the more complex story, and then build on that...gives your audience a chance to get curious and invested in the research itself - and isn’t that sense ...

...to get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check ...personal projects in mind. Thus, to encourage more involvement and provide all our members the opportunity to...previous city-wide genomic studies and our study will involve getting the community to collect various soil samples...

...roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually involves, other...These skills show employers that you can fully investigate and solve a problem or complete a project, overcoming...passionate and capable. For example if the job involves traveling, showing that you are a highly organised...

...researchers, such as postdocs and principal investigators. They also come from many different scientific...scientific research. We continue to support those involved in COVID-19 research, but our new platform Crowdfight...we do in our Frequently Asked Questions. We also invite you to participate in the 1st Crowdfight Symposium...is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the...

...encompasses a variety of technologies to functionally investigate the human genome. Doench says he enjoys working...with RNAi in terms of determining what genes are involved in biological process X, you can do with CRISPR... of individual edits can be studied quickly. Involvement with Addgene Although Doench’s thorough understanding...understanding of CRISPR is what got him involved with Addgene - he says he’s “known of Addgene basically...

...viral production and distribution, this project involved every Addgenie in the company. Our Finance, Business...continues to make improvements to our website and inventory management systems to make way in our systems ...Maintaining our excellent service and accuracy involves caring and careful Addgenies, but also a lot of...Software and IT Teams made major upgrades to our inventory management systems to enable efficient, tracked...

...post, we invite you behind the scenes in our content writing process, and to even get involved yourself...standards. It’s always a work in progress! Get involved A lot of our content already comes from you and...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and tell us...

...to do that we’ve had to navigate the challenges involved in plant synthetic biology. Challenge #1: Public...unique metabolic processes of plants are being investigated on limited funding and time. While institutions... standing behind it. Our color changing flower involves two basic components: the pigments (anthocyanins...research for decades, and the biochemical pathways involved in central anthocyanin metabolism are well understood...

...using the kit to target a large number of genes involved in cardiovascular and metabolic diseases. We are...Addgene: Tell me about the history. How had investigators done this kind of work before? Musunuru: In ...Unfortunately, it is time consuming and difficult for investigators without a lot of expertise to create zinc finger...and puts it in the hands of pretty much all investigators. Our kit makes it extremely simple to design...

... of genes involved in the execution of a specific regulatory regime (such as genes involved in metabolism...unknown. Together, NRs govern expression of genes involved in a broad range of reproductive, developmental...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...

...networking with your local education community. Get involved in education advocacy non-profits like Phipps ...science projects Lastly, if you'd like to directly involve the public in your research projects, you can set...genomes requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload...project. Science communication opens doors Getting involved in science communication has been the most rewarding...

...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience... is now studying how Argonaute pathways may be involved in preventing silencing of self-genes. Dr. Mello...presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families... natural role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile...

...public to access basic equipment and to become involved in developing tools and solutions to research ...makerspace is. The computer scientists can get involved in modelling, the electronics folks can build ... top of that model, and the biologists can get involved a in variety of aspects from the molecular biology... feel free to contact your local space and get involved! Derek Jacoby is a PhD student at the University...

...Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...applications. But how does one invent a tool for applications that don’t even exist yet? Andrew York, Maria...applications are still yet to be demonstrated (or invented), its behavior has already been successfully reproduced... chemistry and protein biophysics, as well as inventors and biomedical engineers, join efforts to explore...

...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

...step and mix the samples well by vortexing or inversion. Created with BioRender.com.   When choosing...curve, change pipette tips and mix thoroughly by inversion or vortexing between each step of the series. ... dynamic range of the curve, then the assay is invalid. If the unknown sample’s measurement is too low...increase the accuracy of the data. For assays involving sigmoidal curves, manufacturers often recommend...

...researching, winning, and effectively managing grants involves much more than writing. In fact, many grant applications...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...organization in a position that required some involvement in the grants process and managing programs that...