...excited to launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll...world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247 plasmids. We... our distributor partners, with the launch of antibodies in China and expansion of viral services available... in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first IPI collection...

...Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies 101: Conventional...of two stains, in this case through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse...

...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression... Plasmid Special Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid ...

...customizable protein degradation. Enter TRIM-Away - an antibody-based system developed by the James and Schuh ...protein variants when used with a variant-specific antibody. TRIM21, an E3 ubiquitin ligase, is the basis ...recruits ubiquitin and the proteasome to destroy antibody-bound pathogens. Clift et al. found that addition...addition of exogenous TRIM21 protein along with an antibody targeting the protein of interest led to rapid...co-microinjection of TRIM21 protein and the target antibody works well. In larger cell populations, you can...-Away, and you only need to deliver the target antibody. TRIM-Away reagents and reporters are available...

... were more resistant to neutralizing anti-AAV antibodies. This is particularly useful for therapeutic ...applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic efficacy of ...particles with membrane vesicles protects them from antibody recognition and inactivation (György et al., 2014...more resistant to inactivation through anti-AAV antibodies. References György, Bence, et al. "Naturally...enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery in vivo." Biomaterials...

... a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol video, ..., "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily be adapted for...

...on the Addgene blog Antibodies 101: Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence...ExM protocols are compatible with conventional antibodies, chemical dyes, and genetically encoded fluorescent...microscopy using fluorescent Fab fragment secondary antibodies. Methodsx 9:101796. https://doi.org/10.1016/j.mex...labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038...Immunofluorescence Antibodies 101: Affinity Reagents ...

...uncharacterized protein, or a protein for which a good antibody has not been developed (and just because your ...your protein has a commercially available antibody, that doesn’t mean it’s a good one), then your first step...depend on your application (see table below). The antibodies available for these tags really are good and ...or DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase ...and polar residues improves solubility; good for antibody-based detection SUMO ~100 amino acid protein...GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags...

...induce hypertonicity display enhanced monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991...Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate...18613082. 3. Oyaas, K., et al. "Hybridoma growth and antibody production under conditions of hyperosmotic stress...osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and bioengineering ...

... single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...They possess many advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble...still just as specific to their target as regular antibodies. These properties make nanobodies easier to transform...

...are a few of the reasons antibodies may result in a lack of response. Antibodies targeting the tumor-augmenting...chemokine production in the tumor microenvironment. Antibodies that block cell-surface tolerance-producing proteins... Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway, VEGF production,...solid as well as blood-based tumors. Monoclonal antibodies have been shown to aide chemotherapy and result...successful elimination of certain tumors. However, antibody stability, half-life, tumor penetration, inadequate... Weiner, George J. "Building better monoclonal antibody-based therapeutics." Nature Reviews Cancer 15.6...Andrew M., Jedd D. Wolchok, and Lloyd J. Old. "Antibody therapy of cancer." Nature Reviews Cancer 12.4...

...on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals who received both doses of the...disease severity and the development of neutralizing antibody responses in patients. They were able to tweak... “In that context, what we showed was that the antibody responses are really not sufficient after one ...Iafrate AJ, Balazs AB (2021) COVID-19-neutralizing antibodies predict disease severity and survival. Cell 184...

...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this...which includes both neuroscience and general-use antibodies, is available in both trial- and full-size, with...

...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...monoclonal antibodies validated for neuroscience research. The plasmids encoding these antibodies are available...Initiative collection of plasmids, viral preps, and antibodies here. Collections...community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN Initiative...plasmids in our collection! These monoclonal antibodies are produced in-house and undergo application-specific...

...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...specificity in antibody-based methods. Antibodies can recognize and bind other antibodies using epitopes...per antibody unit, which is typically the number of antibodies multiplied by two, as each antibody has...These are antibody mimics instead of modified antibodies and are not closely related to antibodies. Monobodies...

...Regenerative Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging...protein complexes - from the cell-lysate using antibodies against the target protein. The captured protein...to find (or generate in-house) a pre-validated antibody for each target protein. To study 20,000 human...frustrating. One elegant solution to sidestep the antibody problem is to tag the protein of interest with...immunoprecipitated using a standard tag-specific antibody (Jarvik and Telmer 1998). Indeed, this method ... of interest, and a universal all-purpose “tag antibody” can be used for downstream IP/ChIP assays. Creating...for both the activity of CRISPR reagents and ICC antibody specificity. Derive clonal lines by single-cell...

...infection. For example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are ...among the human population. These pre-existing antibodies can interfere with virus-cell interactions and...seriously hinder gene therapy outcomes. Anti-AAV antibodies developed by the immune system – patients’ own...own immune systems can develop antibodies that neutralize the AAV vectors used to treat them. Scientists...

...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...

...beads. Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A...Immunoglobulin G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since ...is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior to the ...

...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for...