Skip to main content
Addgene
Showing: 101 - 120 of 955 results

  2. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

  3. Distribution to Industry

    Type
    Collection
    ...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...

  6. AAVED

    Type
    Collection
    ... give a 20 minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation...

  7. Qi Lab CRISPR Page

    Type
    Collection
    ...Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar...

  9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...from 40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...infection, because this setup allows for experimental replicates for each of the gRNAs. The idea was to assign...The SPARC system uses competitive recombination sites to selectively remove a stop cassette upstream of...expressed. By truncating one of the recombination sites in the construct to different lengths, the density... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...

  10. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...

  12. Open Enzyme Collection

    Type
    Collection
    ...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

  13. Ras Pathway

    Type
    Collection
    ...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

  14. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, ...neutralized. The B.1.617.2 (Delta variant) was not tested at the time of this study. Figure 1: Neutralization...cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et al., 2021 with permission...happening.”  In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses...

  15. Pouring LB Agar Plates

    Type
    Protocol
    ...number of plates you’d like to pour. For example: Because we’d like to make 20 plates, and our plates can hold... and negative test results. Sample Data In all cases below (-) indicates that the tested strain is not... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may...Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10...colonies, we recommend using larger plates. Many labs use 100 mm x 15 mm plates which can hold 10 - 15 mL of ...appropriate number of plates and stack them on your lab bench. Label the plates with the date and the ...remainder of the plates, pour directly from the bottle. Pro-Tips Be sure to swirl your plates after pouring...

  18. RNA Extraction Without A Kit

    Type
    Blog Post
    ...your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent ...

  19. DNA Quantification

    Type
    Protocol
    ...readings than a DNA sample dissolved in buffer (such as TE). You will get much more accurate and consistent ...ranges from 1.80-2.00). Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop...
Showing: 101 - 120 of 955 results