Deep Mutational Scanning with One Pot Saturation Mutagenesis
Type
Blog Post
...phage display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate...discover unexpected epistatic changes in protein activity. See Natarajan et al for an example of this.
.... Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale Using Deep...These enzymes, Nt.BbvCI and Nb.BbvCI, recognize the same restriction site, but nick opposite strands of DNA...: The second mutant strand is synthesized in the same manner as the first mutant strand, but a universal...followed by the top strand. Both produced about the same number of positive clones. See Supplementary Note...primers? The key is to design primers that are the same sense as the strand of DNA degraded in the first...