... Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, ...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (...

... encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when we...fluorescent proteins has allowed researchers to make innovative tools not possible with other luciferases. In...luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...

...Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...

...Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker... talk about work at the Institute for Protein Innovation (IPI) (49:45 - 1:18:45), there are also initiatives...conflict with our original hypotheses. Looking for novelty in our stories, we are additionally averse to producing...productive criticisms that focus less on perceived novelty and impact. Journals could also begin requiring...

...-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously synthesized and ...virtually impossible to determine specific protein turnover or temporal expression using standard FP-chimeric...stability, chromophore maturation rate at 37 °C, turnover rate, and the level of background fluorescence...scientists can track single protein localization, turnover, and trafficking, as well as organelle trafficking...

... a diverse range of perspectives and sparking innovative thinking that may benefit your current project...mindset that can lead to unexpected discoveries and innovative solutions. Allowing for serendipity in your lab...confines of a predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace...

...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...programmable platform well-suited for various innovative biological and translative technologies. Addgene...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

...different viral production strategies or developing novel methods for data analysis, our team’s goal is to...Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha, 2006) and retroviral production ...phase, but not the production phase, improves adenovirus production." Journal of biotechnology 157.1 (...

...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional...

...Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...of EvolvR The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant...spectinomycin, rpsE. These results not only uncovered novel resistance genotypes, but also provide a better ...

.... M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V., . . . Lukyanov, K. A...Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other photosensitizers used for cell ablation...

...is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost...     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000 year history...undergraduate degree in English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been...

...She is particularly interested in exploring how innovative tools influence the direction of scientific research...    Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management...performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/...

...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time...

...; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected use of ...creating new forums and opportunities for open innovation in plant synthetic biology. In our case, we selected...off patent, or not patented, and building our own novel pieces where applicable. We will also be working...

...includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for...using these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally...Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two ...Lin and Ryohei Yasuda have teamed up to develop a novel monomeric cyan-excitable red fluorescent protein...

...one, is a sterling example of collaboration and innovation between legal and scientific fields.” Thanks ... every day for their dedication, flexibility, innovative thinking, and openness to team work.   Company...positions. We have an awesome talent pipeline. Turnover at Addgene is about half of what is standard in...

...Research Strategy highlights i) the significance and innovation of the project and ii) how you, the expert, will...writing a proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary...funding. Convince the readers why and how your innovation will address a variety of challenges and impact...

... in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The least ...is usually classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate...integrate into the host’s genome. However, some adenovirus strains are known to cause respiratory diseases ...

...replicate itself and depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient...a “helper-free” system, in which the required adenoviral helper genes are supplied on two different packaging..., J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...