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We narrowed to 150 results for: LYC

Showing: 101 - 120 of 150 results

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Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin screen...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

...unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic amino ...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

... usually 15-20 amino acids long and made up of glycine and serine with dispersed hydrophilic residues ...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...post-translational modifications on those proteins, like glycosylation patterns. Surface labeling is particularly useful...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...are directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands...
Hot Biosensors 2022: Year-End Roundup

Type

Blog Post

... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this approach...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...ataxia telangiectasia, congenital disorder of glycosylation, and Niemann-Pick disease type C. Although the...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG If you need more...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...acids1 AAV receptor2 AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (not strictly required)...
Quick Guide to All Things Lentivirus

Type

Blog Post

...protocols you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol...
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Type

Blog Post

...various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently attached to...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

...liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5): p. 568-72. PubMed PMID...
How to Deposit Your Plasmids with Addgene

Type

Blog Post

...conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify...
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

Type

Blog Post

...further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein...
Institute for Protein Innovation

Type

Collection

... Glypicans are a family of highly conserved proteoglycans crucial for regulating cell signaling during...
Using AAV for Neuronal Tracing

Type

Blog Post

...Hirokawa, K.E., Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka...
SARS-CoV-2 Pseudotyped Virus

Type

Collection

...virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped virus particles...
CRISPR Plasmids - Base Edit

Type

Collection

...that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived...

Showing: 101 - 120 of 150 results

Results per page:

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

Antibodies 101: Beyond Surface Labeling

An Integrin Antibody Toolkit from IPI

Hot Biosensors 2022: Year-End Roundup

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Treating Muscular Dystrophy with CRISPR Gene Editing

Antibodies 101: Multiplex Immunofluorescence

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Quick Guide to All Things Lentivirus

Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

How to Deposit Your Plasmids with Addgene

CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

Institute for Protein Innovation

Using AAV for Neuronal Tracing

SARS-CoV-2 Pseudotyped Virus

CRISPR Plasmids - Base Edit

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