...time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies...

... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

... repositories like Addgene provides large-scale savings. How large? Using Doench’s calculations and the...

...sequence, or if you want to amplify sequences from a large genome organisms like mouse or human. There are ...difficulties in amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...

...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...

...input to an RSM drives expression of a distinct large serine recombinase that recognizes its own attB ...high copy, ColE1 origin of replication), contains large serine recombinase genes downstream of inducible...faster or more efficiently than others. Multiple large serine recombinases have been described in the literature...

...affinity reagents is their size. Antibodies are large enough that their bulk can be a barrier at times...difficult to source, may not be readily available for a large number of targets, and can require specific conjugates...

...guanidine hydrochloride, as a potential treatment for a large number of rare genetic diseases. Spontaneous mutations...that cause rare diseases. They now plan to test a large number of other disease-linked Arg-mutant proteins...

...the advent of CRISPR/Cas9 technology, inserting large tags into a precise location in the genome is still...fact. Later, when we transfect the cells, these large regions of homology enable us to effectively utilize...

...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

...strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this blog,...this sounds really neat — but when is this type of large-scale assembly useful, you may ask? You might be...put, it assembles individual DNA components into larger units or circuits with functional relevance following...MoClo enables individual parts to be assembled into larger functional units which are transcribed together...translational output, etc., which can support a larger cellular engineering goal, several of which are...

...genome edits, but it is effective at wiping out large regions of DNA and has anti-viral application potential...facts – PAM requirement: AAG or TTC  Best for: very large deletions.   Cas12a – The staggered cutter  Originally...

... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...

...luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase is small, bright, and ...

...evolutionary similarity. These are grouped into two large classes based on the structure of the effector complex...

...absence of deletion, the “deletion band” is often too large to efficiently amplify. Typically use primers at...identified with the desired deletion and move to larger plate or flask for growth. Validation of Biallelic...

... some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping Beauty and piggyBac...payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, ...transposons to allow for the targeted delivery of large DNA cargos (>100 kB) to specific locations in the...