Skip to main content
Addgene
Showing: 101 - 120 of 929 results

  1. AAV Packaged on Request

    Type
    Collection
    ...as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and titer viral...research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository...vivo testing. We recommend that you consult the original paper of your chosen AAV plasmid for more information...

  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or frt...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene ...

  4. Viral Vectors

    Type
    Collection
    ...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013...

  5. Open Enzyme Collection

    Type
    Collection
    ...9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

  6. COVID-19 Resources

    Type
    Collection
    ... found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 ... Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-... Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is..., Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

  8. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate scientific research...

  9. TALEN Expression Vectors

    Type
    Collection
    ...assembly are different among the three methods, the final products are the same). All expression vectors listed...Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287...

  10. EXPRESs plasmids for human erythrocytes

    Type
    Collection
    ... collection plasmids for expressing secreted recombinant proteins from human erythrocytes that was created...bio-CD4d3+4-APMAP 73092 bio-CD4d3+4-TMEM24 73093 GlycophorinA-CD4d3+4-bio 73094 GlycophorinB-CD4d3+4-bio 73095...

  11. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Catalytically inactive CRISPR plasmids for purification of target loci.... molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the...

  12. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...

  13. FlyCRISPR

    Type
    Collection
    ... can mediate gene replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro...51026 U6-BbsI-crRNA : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...a ntiviral de fense) complex, comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms ...

  16. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN...tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate dehydrogenase...

  18. Adenovirus Plasmids

    Type
    Collection
    ...the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells...Strain Description PI 16398 BJ5183 Strain for recombination between shuttle plasmid and adenoviral plasmid...

  19. CRISPR Plasmids - Yeast

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

  20. Microbiology Resources

    Type
    Collection
    ...and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...
Showing: 101 - 120 of 929 results