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  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post
    ...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme variant tag that covalently reacts...maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like...Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell ....1016/j.bbamem.2013.09.005 More resources from Addgene Fluorescent protein plasmids and resourcesA practical...
  2. Reflections and Looking Ahead

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    Blog Post
    ...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...
  3. Bringing Sustainable Practices to the Lab: Innovations

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    Blog Post
    ...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...
  4. Easing the Protein Purification Process with pCri

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    Blog Post
    ...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...
  5. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    Blog Post
    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...
  6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    Blog Post
    ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...
  7. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ...further consolidate these FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the ...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...
  8. Intro to Our Summer SciComm Series!

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    Blog Post
    ...communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science writing, ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers...AP biology classes Creating a podcast about genetics using dog breeds as an example adults 45-55 yrs... you next time!  Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication...
  9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post
    ...Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...not permeate through your tissue samples as homogeneously as a single antibody. Using a secondary antibody...10.1177/38.7.2355173  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...
  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post
    ...Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...need to get there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How ...
  11. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    Blog Post
    ... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...The contents of this post are intended to convey general information only and not to provide legal advice...you think about your career (and your future in general), keep in mind that you may have years to work ...www.casseuslaw.com.    Additional Resources on the Addgene Blog Transferable Skills Guide 6 Steps to Submitting...
  12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...
  13. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... [the] academic workforce is pyramidal/feudal generating too many trainees per PI” “Lack of awareness ...Dinman, Chair of the Cell Biology and Molecular Genetics Department at University of Maryland College Park...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...
  14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post
    ...Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages...
  15. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ....1371/journal.pone.0038313 More resources from Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies...
  16. Design Tips for Prime Editing

    Type
    Blog Post
    ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...
  17. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Gelbart, PhD - Director of Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...
  18. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...study binary protein interactions. However, FRET generally only tells you if two proteins interact and cannot...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...
  19. The Scientific Conference Poster Session: Tips for Success

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    Blog Post
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...
Showing: 1001 - 1020 of 1142 results