...partner with Addgene. What are the most important issues for our partners and how can Addgene work with ...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...Overall, spectral flow cytometry has the ability to generate incredible amounts of data from a single experiment...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

... overexpress a recombinant protein? It is not generally in a cell’s best interest to overexpress a protein...for affinity and purification An affinity tag, generally a relatively small sequence of amino acids, is...interest would eventually get ridiculous (you generally don’t want more tag than protein), 2-3 tags is...using plasmids found in this paper (available at Addgene).   Table 2: Protease recognition sites commonly...purification is provided by GE Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent...

...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection... it also has several drawbacks. Direct ELISAs generally have higher levels of background staining. Antigen-containing...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...

... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to...and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...We’re big fans of all our depositors here at Addgene — your contributions make the repository what it... designing a novel topology, then iteratively generated 3D models and amino acid sequences that got closer...Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution Ras...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...

...signs of holiday spirit still linger on around the Addgene office as we peruse the #DeckTheLab submissions...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating...

...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn... I heard an impressive talk about the use of optogenetics tools to inhibit epileptic seizures in a mouse...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...

...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.... During FACS, the sample stream oscillates to generate droplets that are charged as they pass through.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...fluorescent reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...