...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made...

... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...expression in mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian Plasmid Gene/Insert Promoter...Insert Promoter Selectable Marker PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI...

...targeting endogenous CXCR4 gene 46920 pTDH3-dCas9 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused ...controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused ...integrated into HEK293 genome 46922 pSNR52-sgTEF1 Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...targeting endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...

... gRNA_DNMT3b A gRNA to DNMT3b Yeast System: Table 2 To function in yeast cells, we designed Cas9 protein...codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter 43804 p415-GalL-Cas9-CYC1t...codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter 43803 p426-SNR52p-gRNA.CAN1...expression plasmid for use in S. cerevisiae (budding yeast) from the SNR52 promoter Orthogonal CRISPR/Cas9 ...

... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ... ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do...

...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...

.... Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion of...

...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...

...constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination...

...3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina N/A 1 Varies ...Lander 3rd 10 188,509 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast Tavazoie N/A 6-12 ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Inhibition Base Editing Species Human Mouse Fly Yeast T. gondii E. coli S. pneumoniae M. tuberculosis ... CRISPRi v1 – barcodes 181005 161769 Inhibition Yeast Ingolia NA ~6 44,580 KSHV Tiling Library 180272 ...

...comparison to other display systems using phage and yeast (109-1010 members). This selection step involves...

...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don...

...It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ...

... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers...

...optimized for yeast Gal1 Yeast Sandmeyer 26853 pBF3060 Cre codon optimized for yeast Gal1 Yeast Sandmeyer ...mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1 Cre expressed at low levels mutant TEF1 Yeast Piper 60932...mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15 Cre expressed at low levels mutant TEF3 Yeast Piper 61391...TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to the human Estrogen Binding Domain (EBD) Tdh4 Yeast van...-EBD Cre-EBD - Estradiol-dependent control Gal1 Yeast Gartenberg 50797 pUAS-Cre Cre UAS/Hsp70 Mammalian...recombinase CAG AAV Bruchas 78398 pNXRVa-HACre Cre CMV Yeast Tomita 82587 pME-ERT2-Cre-ERT2 Tamoxifen-inducible...

...Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium...

...available for many expression systems: bacterial, yeast, and mammalian. These plasmids contain both untagged...

...organisms for expression in prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth...

...specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms...

...antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and even plants! The zeocin resistance...