...cells after the plasmids have been introduced by transfection or electroporation. All you do to create a protein...

...plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for these methods...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,...

...of cell types that are typically ‘difficult to transfect’, such as primary cells. They can also be directly...

...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein...

...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy...

...an unregulated fashion and thus a marker for transfection. The other (mKate2) is regulated in the 3’ UTR...

...PPI) studies in plants that rely on transient transfection systems using Agrobacterium (such as Nicotiana...

...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...

...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

...selection or fluorescent gating of transiently transfected cells. Co-expression of both plasmids in HEK293...

...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...

... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...

... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

...cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day 7 (am): Harvest cells Equipment Class...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...Polyethylenimine (PEI) 25 kDa MW Pro-Tip Other transfection reagents may be used in this protocol, but their...their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing ... Pass and plate the cells the day before the transfection. Thaw a new vial of cells after 30 passages....to the incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed...Note that longer incubation times can reduce transfection efficiency. Add the OptiMEM + DNA + PEI solution...