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Showing: 81 - 100 of 134 results

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Sleeping Beauty Awakens for Genome Engineering

Type

Blog Post

...and one set of vectors for inducible expression (pSBtet derivatives). The constitutive and inducible expression...
Management for Scientists: What Makes a Good Manager Anyway?

Type

Blog Post

...managing people: Skills for New Managers by Morey Stettner Successful Manager's Handbook: Develop Yourself...
Hot Plasmids and Viral Preps - September 2021

Type

Blog Post

...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...
Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Type

Blog Post

...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...
Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Type

Blog Post

...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...latter experiments, such as transcription factors tethered to dCas9, require long time scales with constitutively...
New and Upcoming Viral Vectors - June 2019

Type

Blog Post

... AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

...prone to aggregation in cells compared to the tetrameric Dronpa it was derived from. What are photoswitchable...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...
Plasmids 101: Cre-lox

Type

Blog Post

...orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break occurs within the core...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

...Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies,...
Split Fluorescent Proteins for Studying Protein-Protein Interactions

Type

Blog Post

...in N-terminal fragment Constant rate of p53 tetramerization in response to DNA damage controls the p53 ...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: ...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...spinal cords by combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years ...

Showing: 81 - 100 of 134 results

Results per page:

Sleeping Beauty Awakens for Genome Engineering

Management for Scientists: What Makes a Good Manager Anyway?

Hot Plasmids and Viral Preps - September 2021

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Improving Transduction Efficiency with Exosome AAVs

Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

New and Upcoming Viral Vectors - June 2019

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Lighting Up Cell Signaling with Photoswitchable Kinases

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Plasmids 101: Cre-lox

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Popular Retroviral Vectors and Their Uses in Scientific Research

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Split Fluorescent Proteins for Studying Protein-Protein Interactions

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Light Sheet Fluorescence Microscopy

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