... Fig. 1 CRISPR activator and repressor mechanisms Want to transiently induce expression? Check...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein...

...Prokaryotic termination   Prokaryotic termination mechanisms fall under two general categories: rho-dependent...dependent, Polymerases I and II employ different mechanisms to terminate transcription. Polymerase I uses...

...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins...

...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

...also provide interesting insights into disease mechanisms and protein function. Getting ready to determine...fluorescent microscopy - even in living cells and whole organisms. You can take advantage of the many Fluorescent...

...Disable the other repair mechanisms Mammalian cells have 2 primary repair mechanisms besides HR - Non-homologous...

...in contrast, engages existing gene regulatory mechanisms to block activity and does not work easily in...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...

...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...cells to understand the genetic and biochemical mechanisms behind their distinct phenotypes and to design...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

...many have mechanisms in place to prevent the burden from getting overwhelming. These mechanisms include ...

...developed for tracking various cell types and organisms and for improving the level of detail possible...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...

...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...He currently works to understand pathological mechanisms of Alzheimer’s disease by developing novel iPSC-based...

...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

...expression. (a) Several engineered capsids with unique tropisms are now available. These include variants with...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species..., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated in parentheses...

...genomes. A) Components of the INTEGRATE system. B) Mechanisms of RNA-guided transposition with INTEGRATE. The... Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac...

...and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

...known is that different serotypes have different tropisms but, as you can see, AAVs of each serotype can...Adeno-associated virus serotypes 1, 8, and 9 share conserved mechanisms for anterograde and retrograde axonal transport...