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We narrowed to 254 results for: sms

Showing: 81 - 100 of 254 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... Fig. 1 CRISPR activator and repressor mechanisms Want to transiently induce expression? Check...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein...

  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...Prokaryotic termination   Prokaryotic termination mechanisms fall under two general categories: rho-dependent...dependent, Polymerases I and II employ different mechanisms to terminate transcription. Polymerase I uses...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

  12. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...cells to understand the genetic and biochemical mechanisms behind their distinct phenotypes and to design...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...

  18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...numerous variants have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...expression. (a) Several engineered capsids with unique tropisms are now available. These include variants with...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species..., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated in parentheses...
Showing: 81 - 100 of 254 results