...genetic variants and disease associations. Plasmids This plasmid collection contains codon-optimized ORF .... Browse the SLC plasmid collection. Depositor... Depositor Collections Resolute RESOLUTE Plasmid Collection You may...Brain Initiative Collection NeuroMab Collection Plasmids Pooled Libraries Feedback Solute carrier proteins... range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR...the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions...

...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...Addgene Browse the repository Find educational resources at Addgene Search for plasmids at Addgene ...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene..., and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...

...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles... 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but be ...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169... to the Addgene blog to get all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox...

...sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps and sequences... we use SnapGene for plasmid quality control are: To identify important plasmid features To align NGS...design experiments for further plasmid verification Identifying plasmid features While our quality control...control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always...send the plasmid off for Sanger sequencing.     If a repetitive region is present in the plasmid, we might...the Addgene Blog How to verify your plasmid Check out our Plasmids 101 featured topic page Additioinal...results to perform quality control on deposited plasmids. We’ve also talked about how our new Snapgene ...

... Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology ...around the world choose to share their plasmids through our repository since we know biological resource centers... indicates that a plasmid has been requested 20+ times   A red flame means a plasmid has been requested...answering plasmid requests can be a time-consuming chore for labs that have popular plasmids but lack ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...

... the successful, internationally renowned plasmid repository that it is today. Although I wish I could...music playing all night was hand-picked (like our plasmid samples!) by Addgenies. If you were there, you ...Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists, part of the Addgene... complicated process of requesting and sharing plasmids has become simplified through Addgene. Looking...Check out our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside ...

...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...components. Networks and Gene Regulation Browse plasmids containing both naturally occurring and synthetic...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...fluorophores. Find all of the nanobody toolkit plasmids here. Buser DP, et. al. PNAS 2018. PubMed PMID...

...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site specific...retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... you can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...

...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...HyPer3 (2) which he will co-express together with a plasmid encoding glycine oxidase. The goal is to determine... develop technologies to protect fresh water repositories in Mexico. At Addgene we're happy to be able...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...

...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...hope every time you come to Addgene to find the plasmid or viral vector for your next experiment, that ...

...materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ID numbers associated...identify a plasmid available through Addgene and find verified information about each plasmid item. Given...associated plasmid page on the Addgene website and find specific information about the plasmid: name, description...description, how the plasmid was generated, validated sequence information, and more. The plasmid page also helps...publications that have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked...specific plasmids used. RRIDs and resolvable compact identifiers now exist for all Addgene plasmids! In an...our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down to the bottom of this plasmid’s page you will...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular... the most frequently requested vectors in our repository and will discuss some of the features you may...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

...about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was...inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available through...other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies...controlled easier than formation of GST dimers. Avi-Tag plasmid for improved biotinylation selectivity A common...exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein of interest...and a 6X HisTag for affinity purification (see plasmid map and cartoon below). Including biotin in your...

...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...to Industry Featured Collections Search Plasmids Browse Plasmids Browse Antibodies Ordering and MTA Information...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ... ready-to-use recombinant antibodies made from plasmids in our collection! These monoclonal antibodies...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...editing complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that...

...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the...

...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...