...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors...vectors. These systems can be used to express multiple gRNAs from a single plasmid with or without expression...expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids Expression...Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease... available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single...Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular locations. PLoS One. 2012;7(11):e49371...Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP Multicolor palette of ATP indicators (MaLion) RGB-color ...

... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy...Nicolas Fawzi 98657 hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2...hnRNPA2_LC_D290V HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98659 hnRNPA2_LC_D220V HNRNPA2B1...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters....Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Examples of intersectional cell population...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations.... This approach has been broadly applied using multiple recombinase-expression ssitrategies to define ...2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72...

... markers, respectively, or constructs in which multiple sgRNAs may be expressed from a single plasmid....non-deletion and deletion bands ( Figure 2 ). Consider multiplexing the “deletion” and “non-deletion” PCR primer...primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells...amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones for Deletions ...μl. This facilitates screening by allowing a multichannel pipette to be used for the remainder of the ...in each well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for...agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject each...

...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

... activates the expression of downstream genes. Multiple sgRNAs targeted to the same promoter act synergistically... collection of reagents for CRISPR/Cas9-based multiplex genome engineering in diverse cell types. This...This set of plasmids is described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral...

...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell... Stem cells are classified as pluripotent or multipotent, based on their ability to generate different...related cell lineages. Pluripotent embryonic and multipotent adult stem cells have been studied for over 30...

...community. Key features: Allows for visualization of multiple proteins simultaneously with dual labeling when...proteins Single constructs are compatible with multiple applications (different fluorophores, biotin, ...George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface proteins. J Am Chem Soc...

...p53 also promotes aberrant mTORC1 activity, and multiple familial cancers are caused by inactivating mutations...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

...Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic markers,...Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - ...Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism...

...cut S. pyogenes 26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799... pyogenes 25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325...23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang.... pyogenes 23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun...

...particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically called packaging...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction...plasmid (pGIPz based) for inducible knockdown, multicistronic design enables tracking of viral transduction...

...experiments for multiplexed targeting with Zhang lab plasmids? For multiplexing CRISPR to target multiple genome...

...neurons with multiple distinct colors Mammalian Sanes See article Multibow constructs Multibow uses a binary... to facilitate Cas9 mediated genome editing in multiple tissues. FLP/FRT: Alternative recombinase system...

...Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific multiplex gRNA plasmid Bullock...

... effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned...empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids for In Vivo Imaging...

...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for...contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions...

...consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human Doench 3rd...

...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...