RNA Extraction Without A Kit
Type
Blog Post
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Allewell NM, Sama A (1974) The effect of ammonium sulfate on the activity of ribonuclease A. Biochimica...in cells, and without something to block RNase activity upon cell lysis, RNA is degraded. To effectively...-based solutions that work by inhibiting RNase activity within cells or tissues- they don’t actually chemically... RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease...tissues, cells, plants, fungi, or bacteria, keep samples cold and work quickly to mitigate RNA degradation...using DEPC-treated water, autoclave the water to inactivate the DEPC.
Ensure that plasticware or glassware...180°C for at least 4 hours.
If your final RNA sample(s) are resuspended in water or TE buffer, store...