...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...

...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

... Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. ...well suited to directed evolution applications. Examples of these base editing systems include targeted...

...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links...

...uide RNA (sgRNA) designed with a 20-basepair complementary region to any gene of interest. Co-expression...or PAM, sequence: NGG) juxtaposed to the DNA complementary region. The uniqueness of CRISPRi, as compared...

...transfer plasmid, including legal approval and an implementation letter DNA amplification Viral vector production...researchers with troubleshooting and results that complement published data, capturing the insights that often...

...for the gene, or browse the gene list below. For multiple isoforms or subunits, individual links to each...available plasmids for the gene. For components with multiple isoforms or subunits, individual links to each...

...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...scaffold has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM Lentiviral plasmid expressing dCas9-2xAM...2xAM and gRNA cloned in Bbs I sites. 92221 pLenti_dCas9-2xAM_hIRF-1 Lentiviral plasmid expressing dCas9...

...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TTTCCTCTCATAGTCAATattaattctctatttgGCTCAAAATGTCAGTAAA Plekho1b-1 TAL3142 & TAL3143 TCGTCAAAGCGGGGCCCTcaggatgccaatcaacAGCCTGTGCAGCCCGACA...

...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...or somatic) stem cells, can differentiate into multiple cell types, but are typically limited to related...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...can bind to the mRNA (hence, the anticodon is complementary to the codon), the amino acid from the tRNA ...typically expressed from a single plasmid, with multiple copies of the tRNA. Applications By making small...

...plasmids you would like to see added to this list, please email [email protected] . Synthetic Biology Plasmids...

... related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways Efflux...

...genetic circuits, and higher level gene networks. Examples Include: promoters and terminators repressors ...

...receptor bait and prey plasmids as a positive control. Please see the following references for more information...