Pushing the Limits of DNA Assembly
Type
Blog Post
...desired mutations. This same process can be used simultaneously for the generation of one or more mutations...
We narrowed to 181 results for: Lta;
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Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...compartment, you could express them individually or simultaneously. Do note for control purposes that there are... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...here is that, as more samples are sequenced simultaneously, it gets harder and harder to obtain balanced... -
DAP arrays for multiplex gene editing
TypeBlog Post...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...domain and a nanobody. AsLOV2 is a light-oxygen-voltage sensing domain from the oat plant (Avena sativa... -
A Deep Dive into BioBuilder
TypeBlog Post...Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education and education... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... vivo, transfection, viral transduction) for simultaneous multi-gene editing with a single crRNA array... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...allowing multiple proteins to be visualized simultaneously. Complementation systems for examining protein-protein... -
Viral Vectors 101: Biosensors
TypeBlog Post...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed... -
Viral Vectors 101: Viral Applications
TypeBlog Post...Libraries allow you to test thousands of elements simultaneously in your system instead of just one at a time... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...fetal hemoglobin gene, ABE8e made both changes simultaneously with 54% efficiency, about 7 times higher than... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning... -
Genetic Code Expansion
TypeCollection...LeuRS E. coli Mammalian Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14 TrpRS-h14 (S8A, V144G, V146C) E. coli... coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9 (S8A, V144S, V146A) E. coli... RF1 function removed Michael Jewett 73581 C321.deltaA (Isaacs lab) all TAG sites changes to UAG, RF1 ...distinct non-standard amino acids. Derived from C321.deltaA, by replacing 1195 TGA stop codons with the synonymous... -
CRISPRainbow and Genome Visualization
TypeBlog Post...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post... and spectral properties, must be optimized simultaneously in order to generate a channelrhodopsin that... -
Antibodies 101: Affinity Reagents
TypeBlog Post...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004).... -
Antibodies 101: Conjugation
TypeBlog Post...M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and transcriptome measurement in single...