... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

...plasmids as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later...that plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify...

...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR...

...position as an Outreach Scientist where I spend a large amount of my time meeting with and talking to graduate...experience is key to my current position, I think it’s largely my work developing myself as a science communicator...

.... Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads...

...solution that fits the most labs, our team conducted a large study which focused on the adoption of ELNs in labs...talking about ELN costs and limited budgets, “a large percentage of survey respondents indicated that ...support that. In addition, if you are working for a large institution or company, site licenses might be available...

...to efficiently deliver large repair templates for applications that require large gene fusions. The current...Matthew H. Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports...

...guides directed towards that gene as there can be a large difference in guide potency. Thus, in cases where...pathways and genetic interactions. Additionally, large scale CRISPR-based screens can be used to gain insight...target effects are generally not regarded as being a large problem for Cas9 activators. This is believed to...makes it much cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based...

...more efficiently amplified than larger constructs, but plasmids as large as ~6 kb can be amplified fairly... This is particularly relevant in cases where a large plasmid with multiple functional regions is used... if you want to introduce a point mutation in a large construct. Particularly, if a pair of unique restriction...visible in a digest of the unmodified plasmid into a larger band (not shown). As a footnote; a similar approach...site, can identify these duplications (slightly larger bands sizes relative to the original template)....

...and to verify the quality of the prep. This was a large factor in Addgene’s decision to launch our viral...category, available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors...were distributed in 2023 alone. You’ll also find a large number of requests for vectors in the “controls”...

...sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach...

...image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein, making...else can you do with the HA frankenbody? Another large advantage of the HA frankenbody is that it remains...

...recommendations from the depositing lab. If your vector is large (>10 kb) or has a low copy number, try following...agar plates, you may notice that there are both large and small colonies. Bacteria containing the recombined...

...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

...optimized by testing various linker configurations in a large-scale functional screen in bacteria. Sensor variants...quite a robust way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied ...

...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...

...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...

...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic...power here is that you could potentially sequence a large number of individuals and find several mutations...

...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...