...log in to your account and check the individual plasmid page to find current pricing information Standard...Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality Control analyses are performed...website. Every order will need to be completed independently including the check out and will require separate...separate shipping charges. Where can I find cloning resources or scientific support? Please see our Addgene...Addgene Protocols for resources you may find helpful. Our Scientific and Customer Support Teams are also...

...plasmids for precisely editing DNA bases without inducing a DNA break....convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted...Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity...base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing...variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution...Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...avoiding known transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

...fused to a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin...it’s briefer time scale and its inducible and reversible nature. Find the LADL plasmids at Addgene Kim...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins... the AID technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova ...specific PAM (protospacer adjacent motif) in order to bind the target sites for editing. PAM sequences can ...they also fused the SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated...

...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress... from. Let’s take a look at some of the stories behind the names. We also have many plasmids in our repository...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however...terrible fate when she pricked her finger on the spindle of a spinning wheel but also a biological transposon...vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the name but some of our...properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval...Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene...

...Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...Alias: CN3987) Expression of jRGECO1a in striatal indirect pathway medium spiny neurons (D2 MSNs) Jonathan...AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs Jonathan Ting Viviana Gradinaru...of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs) Jonathan...

...opens in a new window) The Michael J. Fox Foundation (MJFF) (Link opens in a new window) encourages researchers... table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared Research Tools Labs ...number of Shared Research Tools (Link opens in a new window) , such as CRISPR genome editing tools deposited...table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast... genetically encoded voltage indicators. To expand the voltage indicator toolbox, Ed Boyden’s lab developed...small scaffold proteins to identify novel binding proteins that bind to either the light- and dark- states...cPYP), and plasmids that encode the protein binders (which bind to either the light- or dark- state of the..., because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools ...for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis JM, et al. ...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...

...because the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...additional 'G'. Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer... colonies, then genotype them, it is possible to find both single-allelic and bi-allelic cells. Single-allelic...double nickase system, with PX335 vector, you need to find two guides for each cleavage site, but this system... we usually pick 3-6 guides around the region to find the most efficient guide (most guides would work...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...

... each library can be found on the individual library page. Find Pooled Libraries of Interest Click...pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require electroporation...Knockout Human Sabatini 3rd 10 30,290 Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout... Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout...Mouse Sabatini and Lander 3rd 10 188,509 Yeast Inducible CRISPRi Library – Ampl133 161829 Inhibition Yeast...

...frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->... on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert...homology to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID...

...the ribosome binding site is inaccessible. When the target RNA is present, however, it binds to the switch...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic ...an option, including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol...switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be expressed...display and deep sequencing, revealing that they bind to a much larger subset of protein motifs than previously...biophysical study involving protein stability or binding experiments. Potential experiments that one might...

...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed... homology arms. Notably, they have used Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate..., Flag, YFP, etc) and selection markers. Two independent selection markers are available. An improved ...available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell...

...promoter can be constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA...decreases as RNA is degraded within the cell Short window of CRISPR component expression may decrease off-target...Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target effects...PAM sequence is absolutely necessary for Cas9 to bind target DNA. As such, one can start by identifying...DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation...

... the applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing...applications of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing...interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to study protein-protein...Imaging Image with these powerful tools to study individual plasmids or protein-protein interactions in organs...

...vivo imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue...Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids...-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1 miRFP670...Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation)...

...Link opens in a new window) is a public-private partnership of 13 members from industry and academia financed...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression ...SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...

...method. This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page...following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These...

...Link opens in a new window) PubMed (Link opens in a new window) Article . Individual plasmids can be ordered....g., pmax-DEST [48222] and (Link opens in a new window) Lifetechnologies Gateway Destination Vectors )...

...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ...CMV, PX601 [#61591] ) or liver-specific tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a...