... 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 ...utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 ...