...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...what online tools people generally like to use to share ideas – with the general goal of seeing if and how...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything... sales-y in posts which falls in line with our general mission to share- as opposed to sell- science. ...serious career dilemmas. Some questions were about general advice on how to meet people, how to transition...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based...

...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...examining whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

...engineered ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...utilized yeast display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...

...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex...and 4) generating mAbs against new cancer biomarkers. We believe that repositories, such as Addgene and ...The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: The Expansion of...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...Selecting the Right Antibody Resources on Addgene.org Addgene's Antibody Guide  ...controls for biological experiments, starting with general controls and then moving on to controls for antibody-related...interpretation of results. A positive control is generally a sample or group that will have a desired response...address this, block positively charged groups with generally sticky proteins such as bovine serum albumin. ...Central PMCID: PMC2712964. More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

...into lentiviral vectors to generate a kit which is now available at Addgene. “We called it cloning by ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

...Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky... currently an Outreach Data Curation Intern at Addgene. She is also a graduate student at the University...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional...

...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational...

... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

...Simplifying Plant Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple ...experiments outlined in ABRC’s kits vary by module. As a general guideline, experiments that include growing plants...-12 education.     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic ...

...Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate... Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org...

...lab used an alternative strategy to develop a genetically encoded antibody based probe that detects the...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else..., T., Sato, Y., … Stasevich, T. J. (2019). A genetically encoded probe for imaging nascent and mature ...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...Fluorescent proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...