...in light green; and proteins are represented in orange. Created with BioRender.com.    How do secondary...

...relevant work in your field! However, there’s no guarantee you’ll get a response on the first try (or any...

...results, but this result shouldn’t be taken for granted for all research going forward. It’s just not that...

...vector + insert may spontaneously religate, this transient construct retains functional Type IIS sites and...

...your area. When you are in ‘writing-mode’ for a grant or a scientific manuscript, the reading will likely...

...Development, got her PhD in Plant Biochemistry in France. When she moved to the U.S., she discovered that...

...concentration of any experimental wells in that range of optical densities. If your standard curve does...

...up to 10-12 fragments (Pryor et al., 2020), and randomly selected non-palindromic pairs allows for high-fidelity...

...probably difficult for you to ascertain where on this range your question falls. Here are some things to keep...

...inserted into larger circles or ovals, that are arranged in some logical pattern across the poster. This...

...traditional and social); and synthesizing and translating all these into social media and blog posts, lesson...

...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...

...how best to prepare. Maybe you’re a presentation veteran, but have difficulty answering questions. Or perhaps...

...overwhelming when you are presented with a sea of strangers. Try things like sitting at a table for lunch ...

...the markers in the panel. This complexity index ranges from 0-1, with a value of “0” indicating those ...

...an amide group added to its C-terminus post-translationally, which turned out to be critical for antibody...

...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

...speakers each week, my department and graduate office arranged opportunities to meet with various professionals...

...Gel Transfer Device. Wet the pre-run gel in deionized water and place it on the transfer membrane of the...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last Update...well. Assemble the transfer sandwich as follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top... parameters are correct. Transfer for 5–6 min for proteins <30 kDa. Transfer for 8–10 min for proteins...before running your experiment. Remove the membrane from the transfer device and place it in a container with...Block the membrane in blocking buffer for 1 h at RT on a shaking platform. Wash the membrane 3x for 5 ...Incubate the membrane with secondary antibody for 60 min at RT on a shaking platform. Wash the membrane 3x for...

...of current new and exciting literature. As I transitioned out of academia and away from the bench I certainly...