Addgene

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What's New in CRISPR - November 2021

Type

Blog Post

... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...
Plasmid Tools for Microbiome Studies

Type

Blog Post

...of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome, James Collins...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...study epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety ...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western...
Progress Towards a PAM-Free CRISPR

Type

Blog Post

...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility...
Early Career Researcher Toolbox: Finding Relevant Papers

Type

Blog Post

...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...
A Guide to Designing a Scientific Poster: Content, Layout, and More

Type

Blog Post

...other ways to make your poster more accessible. Final thoughts Though we’ve just presented some standard...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

...microscopy and computer imaging processing. The combination of these methods provides unique opportunities...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...
Transferable Skills: Negotiation

Type

Blog Post

...Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate with...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET (fluorescence...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...transduction or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression system...
Magnetic Control of Proteins: More than a Dream

Type

Blog Post

...possibility that this might just be an artifact and eliminate sources of interference, but the result persisted...
Antibodies 101: Flow Compensation

Type

Blog Post

... you should be able to run your experiment and finalize your compensation values later. This can be helpful...
Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...contain two dimerizing FPs can exist in an open inactive state, a closed active state, and an artificially...

Showing: 881 - 900 of 911 results

Results per page:

What's New in CRISPR - November 2021

Plasmid Tools for Microbiome Studies

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Antibodies 101: Choosing the Right Isotype

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Antibodies 101: Stripping and Reprobing Western Blots

Progress Towards a PAM-Free CRISPR

Early Career Researcher Toolbox: Finding Relevant Papers

Viral Vectors 101: Calcium Sensors

A Guide to Designing a Scientific Poster: Content, Layout, and More

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Open Resources and Plasmid Tools For Studying C. elegans

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Transferable Skills: Negotiation

Which Fluorescent Protein Should I Use?

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Magnetic Control of Proteins: More than a Dream

Antibodies 101: Flow Compensation

Antibodies 101: Conventional vs Spectral Flow Cytometry

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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