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  1. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and with a clean, sterile razor blade, slice the desired DNA fragment from the gel. Notes: To protect the...with the gel fragment. The weight of the gel is directly proportional to its liquid volume and this is ...

  3. Water Bath Protocol

    Type
    Protocol
    ...Set up your water bath so that it will reach the desired temperature by the time you need it for your experiment...water bath to prevent evaporation and maintain the desired temperature. This also helps the water bath heat...

  5. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... likely to ligate to itself rather than to the desired insert. If you are in this situation, it is important...Additional controls are encouraged, but may only be required for troubleshooting failed ligations. The following...

  6. Pipetting Protocol

    Type
    Protocol
    ...maximum possible setting on the pipette, set the desired volume of the liquid on the pipette. Depending ... you can slowly release the plunger. Remove the entire pipette from the container making sure not to touch...

  7. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...to the template DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine... thymine (T)] in an equal molar ratio. It also requires two unique single stranded DNA oligonucleotide...

  8. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Last Update: February 13, 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR...enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use a "Universal...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...proceed overnight at 4 °C if needed. Transfer the entire sample to 3 x 500 mL conical bottles and centrifuge...

  13. Weighing Reagents Protocol

    Type
    Protocol
    ...balance and then tare it. Then add the material directly to the tube to weigh it. Make sure that your scoopula...

  15. Ligation Independent Cloning

    Type
    Protocol
    ...The annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for...

  16. Fluorescence Titering Assay

    Type
    Protocol
    ...the cells in the remaining well, a cell count is required for calculating the titer. Incubate for 48–72 ...

  18. General Transfection

    Type
    Protocol
    ...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

  20. Gibson Assembly Protocol

    Type
    Protocol
    ...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR...
Showing: 881 - 900 of 906 results