...Novartis, Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing...towards researching neurodegenerative diseases.   Additional resources on the Addgene blog Check out all...choosing a lab Resources on Addgene.org Find open positions at Addgene Check out our career videos ...beginning of March, and the jobs are mixed into the general careers section of their web site. Each position... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

...488nm blue light. The T203H mutation in PA-GFP generates a chromophore population that is more neutral,...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't...endogenous protein structure and function. By generating monomeric PA-FPs, there is less chance that the...induce though their continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists...PubMed PMID:18724362. 8.Stiel, Andre C., et al. "Generation of monomeric reversibly switchable red fluorescent...PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium indicators for targeted neuronal...

... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...introduction, we broke down the method into six general steps and outlined the considerations to be made...fixation*. Organic Solvents Organic solvents, generally acetone, methanol, or ethanol, work by dehydrating...to use your specific antibody for IF, look for general IF protocols for your sample type. Starting with...cross-linking fixation; they require an additional step generally referred to as Antigen Retrieval. I chose not ...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

...science communication Resources on Addgene.org Learn how Addgene engages with the community ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...findings, and experiments as articles written for general audiences. So far, we are beginning to make an ...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...BioTechniques.     Additional resources on the Addgene blog Find all of our science communication posts...

...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts...can be created in many different ways, but the general premise is that an aptamer in its unbound state...likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic than proteins. Assays...ions or particularly small molecules that do not generate an immune response. Aptamers can be selected for..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...

...Resources on Addgene.org Find protocols of molecular biology techniques Find plasmids at Addgene Make your...have integrations with a broad range of relevant generic and science-specific tools. In addition, it should...repositories Over the past decade or so a number of general purpose repositories have been launched and are... associated with publications into one of five general purpose open source data repositories: Dataverse...one and preferably more than one of these five general purpose repositories. RSpace has been integrated...@rory_macneil.     Additional resources on the Addgene blog Find tips on choosing an electronic lab notebook...your work more accessible by depositing at Addgene ...

... The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene. gTBE...editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE...this promising start, the team used rational mutagenesis to produce the adenine transversion editor AYBEv3...and ABEs, they could be useful for saturation mutagenesis, and they provide a strong proof of concept for...empty space. Over several rounds of rational mutagenesis, the team progressed to gGBEv6.3, which was capable...s41467-024-50012-w Additional Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors...about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids for base editing Get ...

...publication. Who is to say that the initial data-generating lab has efficiently trawled their own data? A...much time they had. It could be argued that data generators may, in light of the policy, hold onto data longer... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating...amounts of storage. For example, mouse behavioral geneticists may track the motion of multiple animals on different... different occasions via video, generating huge amounts of data. When published, this data is usually ...as SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload...just a single projection of it, could allow idea generation and extension, based on existing, published data...

... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

...communicating science to the general public and training the next generation of science communicators. In...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... is a source of scientific information for the general public as well as a platform for scientists to ...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister... in education, government, and civilization in general (1). Science and politics? Science is an apolitical...as evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link... human consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research...mostly due to human activity while much less of the general public agrees (5, 6). Many of the new administration...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...relatable (17, 20). This is an effort to show the general public that science is done by people like them...

...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...in freely moving mice Record DA dynamics of optogenetically activated and inhibited neurons in live mice...neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396 (2018): eaat4422... PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor enables rapid and ...PMCID: PMC6092020. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...own research project.  3. Do you prefer being a generalist? Working on a variety of projects and in different...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

...include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics continues ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters...to issues with tissue autofluorescence, cell heterogeneity, and/or cell position changes (To et al., 2016...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...exciting revelation. Science in any field can generally be applied to improve our lives and solve global...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...also found that it is more common among first generation students, women, BIPOC, and other minoritized...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career... the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...