Addgene

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Droplet Digital PCR for AAV Quantitation

Type

Blog Post

...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...
The Importance of a Fun Workplace: Company Culture at Addgene

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Blog Post

...place to work, we do SO many fun things!!” Okay - honestly, maybe not everyone replies with quite so much...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Type

Blog Post

...you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...
5 Reasons to Use Reddit for Science Communication

Type

Blog Post

...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...
Hot Plasmids - August 2020

Type

Blog Post

...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...
Antibodies 101: Immunoprecipitation

Type

Blog Post

... Common negative controls include knockout cell lines or tissues that do not express the protein. With...
Hot Plasmids - November 2023

Type

Blog Post

...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...
Viral Production at Addgene

Type

Blog Post

... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...
How to Be an Excellent Trainee

Type

Blog Post

...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...below or browse Addgene’s collection of empty backbones. Our Most Popular Plasmids 101 Posts: Origin...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

...make stricter gates while some like to make looser ones (Figure 3). A more “liberal” gate in the beginning...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

Showing: 861 - 880 of 902 results

Results per page:

Droplet Digital PCR for AAV Quantitation

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Tips from the Repository Trenches: Using Barcodes to Track Samples

The Importance of a Fun Workplace: Company Culture at Addgene

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Twenty Years of Sharing: Addgene's Viral Vector Service

Fluorescent Proteins 101: Aptamer Fluorophores

Changing Labor Laws Bring Increased Postdoc Wages

5 Reasons to Use Reddit for Science Communication

Hot Plasmids - August 2020

Plasmids 101: Plasmid Incompatibility

Antibodies 101: Immunoprecipitation

Hot Plasmids - November 2023

Viral Production at Addgene

How to Be an Excellent Trainee

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: E. coli Strains for Protein Expression

Antibodies 101: Introduction to Gating in Flow Cytometry

Using Phosphoserine to Study Protein Phosphorylation

Thoughts on the Future of Research 2015 Boston Symposium

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