...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

...Since then FRET has also been used to measure conformational changes, cleavage activity using FRET-based...

...MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available to download...window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to assay...techniques. Using a single protein with many available isoforms and mutants provides a shared experience for the...mentor, and more. References for teachers Using Bioinformatics and Molecular Visualization to Develop Student...

...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...genetic engineering and directed evolution. More information about the collection can be obtained at the Open...an expression vector of their choice and then transform into E. coli for expression and purification (... in this collection is ampicillin. Find more information on the enzymes and protocols for expression in...Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 PGD T4 PGD...

...extrachromosomal maintenance and integration of transforming sequences." The EMBO journal 10.12 (1991): 3959...

...proteins from a complex of interest. For more information, read the Split-BioID blog post.  RaPID: RNA-...

... very excited to set up a CRISPR-based editing platform in our lab. Our goal was to use the system to ...

...find personnel salary ranges, go to online job information sites (like, indeed.com, or salary.com). Search...

...recent variants of the Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline...

...and resources are up-to-date with reagents and information you need to use CRISPR in your own lab. Check...Resources CRISPR Guide : Essential background information on CRISPR and the basics for planning your first...to reverse transcriptase install new genetic information into a specific DNA site. Base Edit Base editors...editors precisely edit DNA without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic...

...Packaged on Request Viral Vector Packaging Service Information Production Our production process and quality...viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources ...from the research community demonstrating AAV performance under various experimental conditions. Protocols...institutional biosafety office and your lab, information on the biosafety of viral vectors, and general...

... Collection Technology Plasmids Supplemental Information New England Biolabs offers non-toxic protein ...labeling in a sample. Please see the note below for information on substrates and synthases needed for use of...Control for cell surface localization Supplemental Information SNAP and CLIP tags SNAP and CLIP tag FAQs (PDF... Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic...

...separated by a loop region that facilitates the formation of a hairpin RNA structure, resulting in processing...

...technology. Read the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated...

...genetic information, viruses are a powerful tool for the delivery of this genetic information to cells.... Viruses facilitate the delivery of genetic information to hard-to-transfect mammalian cells. In addition...of different types of viruses. More detailed information can be found in the guides for each virus type...

...Class 2 CRISPR systems called CasPEDIA, with information about enzyme activity, experimental conditions...

...nodes. These methods gave researchers much more information regarding immune cell mobility than the static...

...cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate...

...CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector repeat arrays of ...al., Nat Biotechnol 2012). All three of these platforms assemble TAL effector repeat arrays on a single...Maeder et al., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed...

...been cloned, expressed and validated in recombinant form. The recombinant monoclonal antibodies or R-mAbs...IgG2a 149452 Anti-Kirrel3, short isoform 3 [N320/48R] Kirrel3, short isoform 3 Mouse Mouse IgG2a 149453 Anti-Kv4.2...IgG2a 188208 Anti-FGF13/FHF2, B isoform [N225A/10R] FGF13/FHF2, B isoform Human Mouse IgG2a 188209 Anti-...190541 FGF13/FHF2, B isoform scFv [N225A/10] N225A/10 scFv FGF13/FHF2, B isoform Human Mouse 190542 Pan-Shank...