We narrowed to 916 results for: plasmid

We describe the different classes of optogenetic tools available and what levels of control they give you in your experimental setup.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Protocol for Ligation Independent Cloning

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

Get tips on choosing the best fluorescence microscopy technique for your next imaging experiment.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

Learn about the Green Card process for international researchers and prepare yourself for success in academic and nonacademic science careers.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Lab protocol to determine purity and concentration of recombinant antibodies using ready-to-use biosafe Coomassie stain and ImageJ software.

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.