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  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...

  2. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...elegans embryos. However, this initial timer was a tetramer with a propensity to aggregate in cells (Tsuboi...have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper...

  5. MXS Chaining

    Type
    Blog Post
    ... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...antibody against a target protein is immobilized, or tethered, on agarose or magnetic beads and then incubated...antibody binds to the target protein, thereby tethering it to the beads. Unbound proteins in the mix are...

  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... different induction systems, such as PT7, Prha, Ptet, and Para. The pDonor plasmid pRE57-Ter with multiple... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....

  9. Pouring LB Agar Plates

    Type
    Protocol
    .../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to ...CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest in using...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    ...chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally abundant. Second...
Showing: 61 - 80 of 134 results