...new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely...New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes ...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to...

...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...The Hot Plasmids Segment. Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast... new plasmid technologies from one of our recent newsletters. Listen to the entire Hot Plasmids episode...greatest plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments...articles on the Hot Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If...comments about the Hot Plasmids Podcast Segment, or have recommendations for new Hot Plasmids topics, let us ... comments section below. Hot plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's...

...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...in the repository for many years, has now grown into a collection of over 2,400 plasmids, with many available...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...

...seen through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it...loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding...Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors...FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By Margo R. Monroe Best wishes...detailed information about this new technology. Our Plasmids 101 series continues to grab scientists’ attention...Genome Editing By John Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan ...

... or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational resource...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

...a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...how many plasmids leave Addgene headquarters every day. That would be an average of 673 plasmids each day... without the scientists who deposited plasmids to the repository and for the scientific sharing community...to packing and shipping. Every single day. 673 plasmids shipped daily may be easy to picture (see the ...celebratory lunch, it’s hard to picture one million plasmids. How much space would one million bacterial stabs...post, we looked at how long it takes to make one plasmid and how much time Addgene has saved researchers...

... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing... on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid has been...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested...Citing This Plasmid" list. Email us at [email protected] with the Plasmid Name, Addgene Plasmid ID #, and...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've been...

...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re reading...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download...background.         If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds ...

...switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use in neurons, both in...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...

...insight to the most requested plasmid, the number of requested plasmids, or trends of requests over time...website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is... Visualizing the data So, after downloading my plasmid request data, I started to explore how I could ...file is open for anyone to use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The ...and the source code is available in the Github repository (you can find the link in the upper right hand...

...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide ...Fluorescent Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP...applications of fluorescent proteins and to find the plasmids that are available from Addgene's depositing scientists... of fluorescent proteins (FPs) and to find the plasmids that are available from Addgene's depositing scientists... scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization...genetically encoded fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control...with either actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically...

...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies...conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron...therapeutic applications.  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and ...

...Request service. You can choose a transfer plasmid from our repository and request packaging in one of five ...

... to see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman...stories behind the names. We also have many plasmids in our repository that you can use to study these genes... 1998). Interested in plasmids that contain the groucho gene? Check out plasmids deposited by the Shivdasani... several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however was...website for plasmids that contain tinman or the mammalian homolog Nkx2.5. We also have one plasmid, Addgene...Drosophila Read our Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research ... Beauty (Ivics et al., 1997). We have a lot of plasmids that contain components of the SB transposon system...

... of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your choice of ...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...

...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving away fun Addgene swag for...

...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks... meet our Addgenie crew and sign-up for a free plasmid! Also check out the PLOS Neuroscience Community...

...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...

...here at Addgene — your contributions make the repository what it is. So we’re thrilled this week to congratulate...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution...

...Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven project...