...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue...

...can be a very fulfilling experience; even the grumpiest of researchers can enjoy it at times! What makes...

...Gonzalez-Martinez, G., Alfaro, R., Galián Megías, J. A., Campillo, J., Moya-Quiles, M., Minguela Puras, A., Pons...

...development and testing cycle faster due to the ease of jumping back and forth between running the pipeline locally...

...individual or co- transfection, should be determined empirically.  Negative controls: untreated cells, empty vector...

...Expression of unstable proteins  HMS174 (DE3)** Rifampicin RecA-deficient; allows for cloning and expression...

...research can be patented? I.e. is automated time stamping (detailed records on who did what and when) important...

... education and literacy that this changes.  2) Lumping all scientists (and consequently their results)...

...interest. Thankfully, there are a number of websites compiling antibody data that can help you compare antibodies...

...sequence, based on which ddNTP was added and when. Compiling the identity and location of a given nucleotide...

...associations across different universities are trying to compile more comprehensive demographic information about...

... budget can help you compare the monetary and empirical value that helps you determine whether or not ...

...to make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) ...., a gene whose product confers resistance to ampicillin) is included in the plasmid. These bacteria are... are then grown in the presence of ampicillin. Under these conditions, there is a selective pressure to...Concentration Recommended Working Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin* 100 mg/mL ... *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock solution...

...software has two components - a database of pre-compiled register designs and a search function, both of...

...efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA substrate ...

...hpRNAs, they must be individually engineered and empirically evaluated for their gene silencing efficacy, ...

...Visaggio, D., Gasperi, T., Parisi, M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding...

... can vary lot-to-lot, this range needs to be empirically determined every time you order a new lot of ...

...partner regulatory domains. The Chodera Lab recently compiled the Human Kinase Domain Constructs Kit, a library...

...therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False positives can...