...ultimately leading to the formation of double Holliday junctions (dHJs). dHJs are four-stranded branched structures...resolved via cleavage in one of two ways - each junction resolution could happen on the crossing strand...orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing strand and the other...

...TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP...109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43 Gap junctions 109121 HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone...CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related...

...PMID: 22767332.  3. Kim, Dohoon, Chun-Hyung Kim, Jung-Il Moon, Young-Gie Chung, Mi-Yoon Chang, Baek-Soo...Central PMCID: 4176696. 2. Yusa, Kosuke, Roland Rad, Junji Takeda, and Allan Bradley. "Generation of transgene-free...19337237. PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta...

...results of federally-funded research. 2)      In June, the White House honored 13 “Champions of Change... in celebration of open science. 3)      Also in June, the G8 Science Ministers issued a statement after...

...followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences (TAGC...pic.twitter.com/4MysvpzwkP — Samuel Guay (@SamGuay_) June 18, 2020 Final thoughts on virtual conferences...

...also increased specificity. Most recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9...29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 ...

...dual-vector system, meaning it is typically used in conjunction with a second vector that delivers gRNA, such...lentivirus from Addgene, you could use it in conjunction with gRNA lentiviruses easily made in your own...

...taken up a plasmid and thus should be used in conjunction with selection methods. Combining selection and...screen. Antibiotic selection is also used in conjunction with this method to ensure that positive colonies...

...pic.twitter.com/52KQDMeSjO — Brianna Weiss (@briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks...blogger Joe James from Binning Singletons. During Junior High and High school in the 80s and 90s, Joe was...

...14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes...18784188. PubMed Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome...

... by its Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School/Brigham... Mentorship Program Zafira Castaño Corsino is a Junior Faculty at Harvard Medical School/BWH, co-Director...

...Addgene Podcast. View Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) ...et al. Autophagy. 2008. PubMed PMID: 18094608 JUNE 2016 Repurposing the CRISPR-Cas9 system for targeted...

... celebrating throughout the year, and on Sunday, June 9th, we hosted our 20th anniversary party for all...

...our guest, Wei Leong Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute ...

... launch this summer! Summer SciComm Series From June-September, we’ll be running a Summer Science Communication...

...Kwabena Duedu who were awarded similar grants back in June, Gladys will be using plasmids in creative ways ...

...applications for a second round of plasmid awards on June 15th. As previously, these awards will be open to...

...microbiology and then did some independent research my junior year and discovered I really love science, but ...

..., Joanne had plenty of advice for the group of juniors and seniors, including that it’s never too early...

...continuity - two lead organizers on each program, one junior and one senior.  To learn more about the STIN's...