Zhang Lab's CRISPR Frequently Asked Questions
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...Homologous Recombination (HR)? How long should the homology arms be for efficient recombination? The most...Dr. Le Cong, the first author of a seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a ...Plasmids Dr. Le Cong, the first author of one of the seminal articles from Dr. Feng Zhang's Lab ( Science , ...to learn more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs... to express the Cas9n (nickase). Homologous Recombination (HR) FAQs Can you summarize HR template vector...specific mutations or insertions by Homologous Recombination (HR), what is the length of each homology arm...destroy the BbsI sites, a double digest should discriminate between positive and negative clones. Clones...