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We narrowed to 89 results for: donor

Showing: 61 - 80 of 89 results

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CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

...or to add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, ...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

...Type Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from...
Hot Plasmids - October 2020

Type

Blog Post

... efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce...
15 Hot Plasmids from 2017

Type

Blog Post

...This makes mCyRFP1 a powerful tool for use as a donor in FRET (fluorescence resonance energy transfer)... Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells. Find ShadowR for mammalian cell...
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

Type

Blog Post

...research purposes, with the informed consent of the donor [7]. Adult stem cells (ASCs) Also known as somatic...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

... genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons...
Adeno-associated virus (AAV) Guide

Type

Guide

...AAV transfer plasmids, the first with a 3’ splice donor and the second with a 5’ splice acceptor. When these...
Hot Plasmids Spring 2024

Type

Blog Post

...; v2, vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA, polyadenylation...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors. PLoS One...
CRISPR-mediated Plant Base Editors

Type

Blog Post

...frequencies and relies on the efficient delivery of donor DNA to facilitate repair. Since the development ...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed...
Plasmids 101: Restriction Cloning

Type

Blog Post

... Set up restriction digests for your insert (or donor plasmid) and plasmid backbone. Because you lose ...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

...This allows conjugation between a pir-containing donor and the recipient of interest. Because pDS132 cannot...
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Type

Blog Post

...strand break (DSB) formation and do not require donor DNA, . These precise edits tend to be more efficient...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... Galphai), the C-terminus and N-terminus of the donor fluorescent protein can be truncated to increase...
Cre-lox system

Type

Collection

... components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector Cbh AAV Eisenhoffer 131786 pLenti-CreLite...pAAV-Sox2-CreERT2 Cre-ERT2 - Tamoxifen inducible; AAV donor vector AAV He 135659 pQcre1 Cre PpagA from B. anthracis...
A History of Genome Engineering in Popular Culture

Type

Blog Post

... genome editing without double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038...
Zhang Lab CRISPR Page

Type

Collection

...also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

Showing: 61 - 80 of 89 results

Results per page:

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Hot Plasmids - October 2020

15 Hot Plasmids from 2017

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Fluorescent Proteins 101: Aptamer Fluorophores

Adeno-associated virus (AAV) Guide

Hot Plasmids Spring 2024

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

CRISPR-mediated Plant Base Editors

Adeno-associated Viruses (AAVs) for Genome Editing

Plasmids 101: Restriction Cloning

Harnessing Bacterial Toxins for Allelic Exchange

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Cre-lox system

A History of Genome Engineering in Popular Culture

Zhang Lab CRISPR Page

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