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We narrowed to 70 results for: acr Anti-CRISPR

Showing: 61 - 70 of 70 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific... and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody that...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...that targets your protein of interest or primary antibody respectively. Avidin bound to a reporter is then... on this complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin...

  2. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ...the transfer gene. By dividing the viral elements across multiple plasmids, the risk of recombination and...generally a good place to start. If you don’t anticipate that you will need large volumes of lentivirus...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...with the engineering of fluorescently labelled antibodies made it possible to track cells from one organ...harvesting lymph nodes at different times after antigen challenge, scientists were able to track where ...diverse tissue environments. These studies provide quantitative measurements of cellular motility, interactions...By specifically labeling T cells, B cells, and antigen presenting cells, scientists have been able to ...the bloodstream to invade lymph nodes and scan antigen presenting cells, but two-photon microscopy enabled...scanning was and how fast T cells could jump from one antigen presenting cell to another. Indeed, we now know...

  4. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...quiescent or terminally differentiated cells such as macrophages and microglia. This property makes the lentivirus... method detects the expression of viral capsid antigen p24, the FACS dosing method correlates viral titre...reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have been described...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...in regulating translation and inhibiting cellular anti-viral defenses. Glossary Term Definition Adeno-associated... contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need...wild-type serotype, and a very high infectivity across a broad range of cell types in vivo. AAV-DJ has... capsid could avoid invasive methods such as intracranial injection. Systemic capsids are often derived...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...gene, conferring antibiotic resistance to infected host cells. When these antibiotics are added to the... contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...AIH1, ALGN, AMG, AMGL, AMGX AMH anti-Mullerian hormone MIF, MIS AMHR2 anti-Mullerian hormone receptor, type... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene... ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I AMHR2 anti-Mullerian hormone receptor, type II AMHR, MISR2,...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...including dendritic cells and macrophages. Antigen presentation is mediated by the MHC class I and class II molecules...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...

  8. Adenovirus Guide

    Type
    Guide
    ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...are also being used in cancer therapy to stimulate anti-tumor immune response in different ways, such as...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...specialized immune cells and antibody production that develops after exposure to an antigen. AdEasier-1 cells BJ5183... contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need... verify that the infection rates are equivalent across multiple viruses. The adenoviral backbone plasmid...vectors are excellent vectors for delivering target antigens to mammalian hosts and have been used as vaccines...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...concurrently to minimize the impact of neutralizing antibodies developed in response to the first exposure to...AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will be the capsid ...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...efficiency, and is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...reader) Detect the average amount of fluorescence across a population of cells (i.e. in a plate well). Use... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...
Showing: 61 - 70 of 70 results