...optogenetics plasmids available from Addgene. The popular CRISPR/Cas9 genome editing system has been modified for...vivo genome editing. For more information on AAV-CRISPR, please see this blog post. Browse the article ...serotype; in vivo , it displays very high infectivity across a broad range of cell types. The mutant AAV-DJ8...divided between two transfer plasmids, but with substantial sequence overlap. Co-expression induces homologous...with trans-splicing or overlapping vectors: a quantitative comparison. Duan D, Yue Y, Engelhardt JF. Mol...

... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...with the engineering of fluorescently labelled antibodies made it possible to track cells from one organ...harvesting lymph nodes at different times after antigen challenge, scientists were able to track where ...diverse tissue environments. These studies provide quantitative measurements of cellular motility, interactions...By specifically labeling T cells, B cells, and antigen presenting cells, scientists have been able to ...the bloodstream to invade lymph nodes and scan antigen presenting cells, but two-photon microscopy enabled...scanning was and how fast T cells could jump from one antigen presenting cell to another. Indeed, we now know...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...quiescent or terminally differentiated cells such as macrophages and microglia. This property makes the lentivirus... method detects the expression of viral capsid antigen p24, the FACS dosing method correlates viral titre...reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have been described...

...AIH1, ALGN, AMG, AMGL, AMGX AMH anti-Mullerian hormone MIF, MIS AMHR2 anti-Mullerian hormone receptor, type... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene... ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I AMHR2 anti-Mullerian hormone receptor, type II AMHR, MISR2,...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...including dendritic cells and macrophages. Antigen presentation is mediated by the MHC class I and class II molecules...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...concurrently to minimize the impact of neutralizing antibodies developed in response to the first exposure to...AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will be the capsid ...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...efficiency, and is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...reader) Detect the average amount of fluorescence across a population of cells (i.e. in a plate well). Use... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...