...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...quiescent or terminally differentiated cells such as macrophages and microglia. This property makes the lentivirus... method detects the expression of viral capsid antigen p24, the FACS dosing method correlates viral titre...reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have been described...

...AIH1, ALGN, AMG, AMGL, AMGX AMH anti-Mullerian hormone MIF, MIS AMHR2 anti-Mullerian hormone receptor, type... and Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene... ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I AMHR2 anti-Mullerian hormone receptor, type II AMHR, MISR2,...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...including dendritic cells and macrophages. Antigen presentation is mediated by the MHC class I and class II molecules...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...concurrently to minimize the impact of neutralizing antibodies developed in response to the first exposure to...AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will be the capsid ...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...efficiency, and is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader...reader) Detect the average amount of fluorescence across a population of cells (i.e. in a plate well). Use... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...