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Addgene
Showing: 61 - 80 of 955 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100%...100% ethanol or isopropanol 90% ethanol 70% ethanol TE buffer 3 M Na-acetate (pH 4.8) Protocol: Generalized...the column using water or a neutral buffer such as TE. You will now have plasmid DNA that has been purified... Water-saturated phenol-chloroform can be used if TE-saturated is not available. Vortex microfuge tube... paper towel for 5-20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension...min. Centrifuge the tube for 5 min at 12,000 g. Notes: Pellet contains proteins, cell fragments, salt ...

  2. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...Insert Promoter Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker...

  3. Institute for Protein Innovation

    Type
    Collection
    ...accessible toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant...blocking ligand binding and receptor function Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant...

  4. Chemogenetics Plasmids

    Type
    Collection
    ...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

  5. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation...targeted strand non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...

  7. TALENs for Endogenous Human Genes

    Type
    Collection
    ...target sites in cultured U2OS cells. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites in CAPS...

  8. Synthetic Biology - Overview

    Type
    Collection
    ...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker...

  10. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... length of the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details...two-hybrid reporter system but have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene ...

  11. Microbiology Resources

    Type
    Collection
    ...microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids below...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...

  12. Worm Expression Resources

    Type
    Collection
    ...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing Genomes - Cold...

  13. Mammalian RNAi Tools

    Type
    Collection
    ...interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S, ...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ... transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter PI Publication ...

  15. Lentivirus Plasmids

    Type
    Collection
    ... procedures. Additionally, because this virus integrates randomly into the host genome, it has the potential...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base edit human...Alzheimer’s disease and related disorders. The site curates information on useful resources such as: Antibodies...

  17. COVID-19 Resources

    Type
    Collection
    ...-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397 (Link... Endy and Philippa Marrack Labs. Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Joung ...

  18. Rett Syndrome

    Type
    Collection
    ...window) PMID: 10508514 2007 - Adrian Bird's lab demonstrates that Rett syndrome symptoms are reversible in...Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

  19. Retrograde AAV viral preps

    Type
    Collection
    ...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...secreted version of the protein is available – these attributes have made it popular with scientists since its...luciferase reporter vector via Gateway cloning to test for the presence of transcriptional regulatory region...
Showing: 61 - 80 of 955 results