...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...

...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During this ...

...Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port pDCC6 59985...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI none S. pyogenes...

...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...

...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

...virus). In the absence of tetracycline, the tetR portion of tTA will bind these tetO sequences and the activation...identify which amino acid residues of tetR were important for tetracycline-dependent repression. Mutating...shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping...

...plasmid webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301 cre...intron-lacZ/NLS Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks...

... español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más ...

...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...

...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...

...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...

...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and...

...accessibility in research. For each antibody, we provide important details including the protein and plasmid sequence...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether...PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types...

...Where can I find cloning resources or scientific support? Please see our Addgene Protocols for resources... may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance ...

...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...plasmid. Distribution of the original plasmid is important because it is the actual material being referenced...

...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....