...Core Lentiviral and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector... and copGFP from the PGK promoter Adenoviral Vectors The adenoviral transfer vectors contained within ...Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of...have kindly deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...transformants will instead form white colonies. Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses...

...their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are commonly used as vaccines ...immunogenic response they induce. Some (oncolytic) adenoviruses are utilized for cancer therapy because of their...preferentially infect and kill cancer cells. Adenoviruses have high transduction efficiency and direct...4.5 Kb 18-26 Dividing/Non-dividing No* Very Low Adenovirus Transient double stranded linear DNA >8 Kb 105...Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide University....2010.12.009. PMID: 21167871 Wold WSM, Toth K. Adenovirus vectors for gene therapy, vaccination and cancer...

...Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the Coronaviridae family called...speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like...protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute Addgene COVID-19 Resources...coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR diagnostics:...2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus Information Center The Lancet COVID-...COVID-19 Articles Wiley Online Library - Covid-19: Novel Coronavirus Content Free to Access Collections of...2020, BioRxiv SARS-CoV-2 invades host cells via a novel route: CD147-spike protein . Cathepsin L - Bosch...

...and two-way ANOVAs ANOVA tests can compare either one (one-way ANOVA) or two (two-way ANOVA) independent... form of ANOVA. Isn’t that what a two-way ANOVA test is you might ask? Not exactly! A MANOVA test assesses...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...variation that needs to be accounted for. ANOVA tests ANOVA tests are essentially t-tests with room for...one-way ANOVA can test either the effect of temperature or humidity on crop production.   MANOVA The MANOVA... and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent variables...the “C” that differentiates these tests from ANOVA/MANOVAs. Quantifying and controlling for any covariance...

...viruses originally discovered as contaminants of adenovirus stocks. One major advantage of using AAV for ...the presence of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This ...infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding... Helper plasmid for AAV packaging, expressing adenovirus E4, E2A and VA Wilson RepCap Plasmids for Serotypes...terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production...Maguire 170716 Olig001 Olig001 Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte...

...in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...fact: There are 57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     ...will discuss the gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The components...cell types. AAV also requires some back up from adenovirus genes – E4, E2a, and VA – which are supplied ... within their viral elements. Gene expression Adenovirus is a linear, dsDNA virus naturally. It has ITRs... you wish to deliver to your target cells. In adenovirus, the ITRs serve as a type of origin of replication...of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid components...

...considered if size constraints are an issue. Adenovirus Adenovirus has a linear, double stranded DNA genome...serotypes of human-infecting adenovirus have been identified so far! Adenovirus generally has high immunogenicity...viruses are roomy: some adenoviruses pack up to ~35 kb of space for cargo! Adenovirus is an effective option...used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...mammals, and even the avian population. Some adenoviruses are known to cause respiratory disease across... the other viruses discussed in this article, adenovirus has the highest maximum titer, a plus for production... genomic integration. While the expression of adenovirus cargo is high, it’s generally not as long lasting...

...’s lab in Novartis Oncology.  Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc...research. When she came to Novartis, she was happily surprised that “Novartis was actually really research...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new tool that allows ... and says that she decided to do a post-doc at Novartis because she wanted first-hand experience in industry...investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ...developing more potent ABL1 allosteric inhibitors at Novartis. The ClonTracer library has been distributed all...

...NA E nd A ccumulation during T urnover) : Single-molecule mRNA turnover biosensor that utilizes Renilla... of Luciferase Tools in our Collection Modified/Novel Luciferases AkaLuc : A modified firefly luciferase.... LuxSit-i : An artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase... of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly...

...However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable...Creativity is required to develop new projects and test novel approaches, a necessary skill set for any scientist...ll delve into strategies you can use to foster innovation. If you identify as a non-creative or if you ... by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself a block of...starting point, the ideas generated were hardly novel. When told to use a roller skate as a starting point...but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box concept and ...As a result, the product team is an incredibly innovative group. Our entire company benefits from their...

...scientific teams at the Novartis Institutes for BioMedical Research to develop novel biologics for the treatment...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland.... Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting...Crystal's educational background and position at Novartis. Chapter 2: What's a lab group in industry? The...empower people Chapter 3: How Crystal advanced at Novartis, and how to prepare yourself for success by having... The types of science that Crystal works on at Novartis, how she's learned to prioritize projects, and...listings. Mobility between different positions at Novartis. Chapter 7: How Crystal developed her science ...

...fusion protein inhibitor CASANOVA for “CRISPR-Cas9 activity switching via a novel optogenetic variant of...AcrIIA4.” CASANOVA responds to blue light quickly. Within minutes of inactivating the CASANOVA complex,...Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to ...observed binding telomeric DNA. What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector...genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods...characteristics they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1...technique using small scaffold proteins to identify novel binding proteins that bind to either the light- ...

...Seegers, C. L., Bollen, S., Jore, M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries...control both when and where genome editing happens. CASANOVA , developed by Dominik Niopek’s lab , is a way...It has also been used extensively to screen for novel genes that regulate known phenotypes, including ... maintained by the Jennifer Doudna lab at the Innovative Genomics Institute, University of California,... 35301321 Edraki, A., Mir, A., Ibraheim, R., Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J....30403660 Sanson, K. R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg,..., Gootenberg, J. S., Wu, W. Y., Scott, D. A., Severinov, K., Van Der Oost, J., & Zhang, F. (2017). Multiplex...

...✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation that may optionally...repair system, and more recent prime editing innovations have improved the odds of mismatch repair favoring... C-terminus of Cas9 H840A nickase (PE1). But innovations by the Liu lab and others have led to a variety...base-pair editing window cannot edit within what Fyodor Urnov refers to as “PAM deserts” in the genome. More versatile...cells with 1-10% indels. Since then, further innovations have continued to improve the maximum efficiency...expression and activity In addition to the numerous innovations that streamline the process of prime editing,...reduced editing efficiencies, likely because both innovations aim to solve the same problem. Both approaches...

...members from industry and academia financed by the Innovative Medicine Initiative (IMI) of the European Union...RESOLUTE project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under...the European Union's Horizon 2020 research and innovation programme and EFPIA. Figure 1: Representation...

...this post: Improved voltage indicator: JEDI-2P Novel class of light-gated potassium channelrhodopsins...https://doi.org/10.1016/j.cell.2022.07.013.   Novel class of light-gated potassium channelrhodopsins...labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that HcKCR1 and...photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs represent a long-...performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins are natural...

...urokinase receptor CD87, UPAR, URKR PLXNA1 plexin A1 NOV, NOVP, PLEXIN-A1, PLXN1 PLXNA2 plexin A2 FLJ11751, FLJ30634...receptor - NODAL nodal homolog (mouse) MGC138230 NOV nephroblastoma overexpressed gene CCN3, IGFBP9 NPFF... in early stage tumors-like 1 cdnf ARTN artemin ENOVIN, EVN, NBN AVP arginine vasopressin ADH, ARVP, AVP-NPII...tissue growth factor CCN2, HCS24, IGFBP8, MGC102839, NOV2 CTSG cathepsin G CG, MGC23078 CX3CL1 chemokine (...

...mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). While each...higher end. Adeno and adeno-associated viruses Adenoviruses infect non-dividing cells but they do not permanently...integration causing mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (...non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which means they...cells Insertional Mutagenesis Possible Adenovirus Remains Episomal (Little Risk of Insertional... adenovirus...

...Dumas, Lercher, Spicer, & Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher...engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the UAG codon in...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...experimental design lends itself to creativity and innovation, valuable commodities in any NIH grant application... understanding of your protein that can lead to novel insights about its biology and its behavior. See...in Boston, MA. His interests include developing novel fluorescent tools for labeling reagents and structurally... of ryanodine receptor type 1 structure using a novel site-specific labeling method." PloS one 4.10 (2009...

...did we get here in the first place? How CRISPR innovation affected software development CRISPR, like the...it, offers an interesting view into how rapid innovation affects the research community. I’ve likened ...That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata), and software development has caught...separate sgRNA design tools into database and de novo solutions. Database tools allow us to view and get...community in order to succeed. With respect to de novo sgRNA design, I again separate it into two categories...future of CRISPR software is promising. Rapid innovation in this space has put us in a good position to...