...Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used in research...

...to Addgene's empty vector backbones. For the most part, we will assume that you want to express a ...

...appropriate. If your gene of interest should be mostly inactive, and only turned on occasionally, using...

...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

...TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA prothymosin alpha a TAL3344 & TAL3345 TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA...

...Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss...don’t have the networks or experience to get the most of meeting.  I experienced this at the ASM General...and helping societies help their members get the most out of their meetings. Amanda Gunn ran randomized...

...Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-...so commonly used is that it is good at infecting most cells, but not all. For example, LDLR expression...envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping,...

...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...

...number of cells is limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to...

...gene in vitro and in vivo to use in biosensors. The most common types of luciferase found in Addgene’s collection...

...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...

...GAGTCCGAGCAGAAGAAGAA 69236 cut S. pyogenes 26480473 Wolfe ttTi5605 Mos1 insertion site C. elegans ATATCAGTCTGTTTCGTAA 47550...

...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...

...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...

...Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender U (2025)...Gorantla N, Simkins J, Crespo EL, Ikefuama EC, Tree MO, Prakash M, Björefeldt A, Barnett LM, Lambert GG,...Addgene's collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic...Early Chemogenetic Receptors: RASSLs DREADDs PSAMs LMOs Planning Your Experiment References Chemogenetic... channels targeted to specific cell populations (most often neurons) that can be turned on or off by the...have few, if any off-target effects. They are the most widely employed type of chemogenetic receptors. ...: hM1Dq, hM3Dq, and hM5Dq, however, hM3Dq is the most widely employed. Gi-DREADDs signal through the Gαi...

...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

...pMAZe Reporter construct for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional...

...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...