...mutagenesis for the brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission...mWasabi, a bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP...-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...protein at the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation...changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein The team that developed...Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent...PMCID: PMC2292683. Shaner, Nathan C., et al. "A bright monomeric green fluorescent protein derived from...

...Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific...behind the journal and hear from a fantastic team of high school students who recently submitted to the journal...Journal of Emerging Investigators. Chapter 2 - Getting high school students and teachers interested in JEI (...mentorship programs. Chapter 6 and Outro - Interview with high school student researchers Arooba Ahmed, Jiachen...

...separate proteins based on size. Whether higher molecular weight complexes are detected will depend on the...series described a practical approach to selecting a bright fluorescent protein and a photostable fluorescent... have a natural affinity and a tendency to form higher order aggregates. This property can be tolerated...purified fluorescent proteins to form homodimers or higher order oligomers can be analyzed by several techniques...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...will be using. If you do a head-to-head comparison right, it will be valuable information for the scientific...

...molecule. A higher brightness increases the detected signal and therefore high brightness is an important...EGFP). The higher the number, the higher the theoretical brightness. The theoretical brightness often appears...are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness Brightness refers ...practical brightness is a better criterion than theoretical brightness for selecting the brightest fluorescent...and the sample. Practical brightness A protein with a high intrinsic brightness that does not fold well ...how the brightness is best defined or determined. Here, we will explain how practical brightness is determined...protein. Theoretical brightness Two key properties of fluorophores that determine brightness are the extent ...

...ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing...way to design GGA overhang sets, particularly for high-complexity reactions? The researchers over at New...otherwise! They suspected a data-driven approach might allow for an increase in the complexity. In 2018...need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher? The five ...100% GC.) In fact, the NEB® team’s data showed that high-fidelity reactions could be done using overhang ...sets that broke rules 3—5. Fidelity, it turns out, might depend more on the individual sequence(s) than on...build out three tools to help researchers design high-fidelity Golden Gate Assemblies and overhang sets...

...with the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...expression of light-gated ion channels such as channelrhodopsins (ChRs) makes them light-responsive. When...mm3). Current channelrhodopsins also need high-intensity light for activation which requires implantation... no blue light, the channelrhodopsin remains inactive and neurons don't fire. With blue light, ions flow...are highly constrained and poorly understood. It’s hard to design new channelrhodopsins with high-throughput...requires higher expression levels in order to activate a neuron. ChRgers, however, have a higher conductance...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...

...Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids....

...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

... plasmid with or without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our...Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and...

...a variety of popular plasmids and backbones with high cloning potential. By making these plasmids available...spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations. Can I directly transfect...

... the results, techniques and reagents with the highest-possible quality of research output, RESOLUTE expects...production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated...

...hand, there are reasons why grad school might not be the right choice. I spoke with our Financial Operations...here. You also don’t have to go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was.... I thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date...biggest takeaways from my co-op. It has been so insightful to learn about different career paths, successes...

...oneself that prompted us to create Crowdfight.   Figure 1: Crowdfight is a community of scientists who...making science.   How we started Crowdfight-COVID19 Crowdfight was born as a collaborative platform...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the expert...Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked...did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform the experiments... them heard about our online platform, called Crowdfight, and filed a request for help. In just a few ... a research group in Scotland, who had just the right infrastructure and offered to collaborate with them...

...researchers, we wondered how we could make the insights gained during this time more visible and thereby... ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video to learn ... ground again and again as they don’t save the insights they glean from a reading session. To remember...hunting through a whole folder of papers to find the right experiment! The ReFigure extension also tells readers...ReFigure making it easy for others to discover your insights on a topic. Beyond collating other’s data, you...include your own data in a ReFigure. For example, you might include a confirmatory image to show that a previously...previously used reagent performs well, or you might publish a replication experiment that you conducted ...

...range between 0 and 100) are then converted to a weight (which ranges between 0 and 1) so that they can...ranging from -40 bp to 40 bp) are also converted to a weight (which ranges between 0 and 1) (shown in the graph...Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis of CRISPR experiments...

...increasingly able to harness the body’s immune system to fight progressing cancers. It was inspiring to participate...from all fronts!”  Here I aim to compile a few highlights from the conference which reflect how cancer/...targeted engineering of the TCR locus. He also highlighted the UCSF Immunoprofiler - a human biopsy analysis...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...Larsen 107787 AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Light-Inducible Recombinases ID Name Promoter Fluorophore...

...Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...complete any additional customs documentation that might be required by your country for the import of biological...

...pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling Browse...Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and...

...their large packaging capacity (>8 kb), high titers, and high levels of transgene expression. Additionally...