...Plasmids Find Plasmids by Gene Name Alternative Gene Names Gene Description AR plasmids AR, RP11-383C12.1...and results in activation or repression of target gene expression. Nuclear receptors play a key role in...homeostasis, and the receptors and their downstream genes are associated with numerous disease pathways. In...this collection, email deposit@addgene.org to be paired with an Addgene scientist. Nuclear Receptor Plasmids...collaboration with research labs from around the globe, Addgene has assembled a Nuclear Receptor plasmid collection...

...listed below. When targeting other gene loci, you will prepare the gene-specific CRISPR and donor vectors...multiple genes. Plasmids can be found associated with the following article: Paix, et al. Genetics 2014 Allen...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...repair template design. How to use CRISPR to tag your gene of interest Mendenhall and Myers Tagging System ... the plasmids as listed in each row. If your own gene of interest is currently unavailable, you will need...terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes. This... is different from the conventional HDR-mediated gene knock-in. The detailed procedure can be found in...

... deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF Shared...research. This collection is part of Addgene's Neurodegeneration Research Collection , which includes ...Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools (Link opens ...

...to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 14(3):e7899. doi: 10.15252... CRISPR knockdown to modulate expression of two genes without cross‐talk. (A) Schematic of the strain ...

... choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors. The most...effect of mutations of these regulatory elements on gene expression. Empty backbones for inserting regulatory...an intron in the middle of the Firefly luciferase gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during...into which you can clone your regulatory element or gene of interest into to create a luciferase reporter... Firefly/Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of...enhancer regions. Mutations within the luciferase gene allow for increased copy number in E. coli and expression... Firefly/Renilla Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under the ...

...depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages with information...KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we...

...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi...each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells ...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman...Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini ...Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd...

...page to look for specific genes or applications that are not listed here. Addgene Microbiology Resources ...of Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye...Lab Synthetic Biology BIOFAB plasmid set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular...Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast ...Resources : Browse plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio...Resources for Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Blog Posts Prime editing is a “search and replace” gene editing method in which a reverse transcriptase ...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...plasmids for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert Promoter... target a specific promoter or enhancer for your gene of interest. Available modifications include: histone...Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...

...vectors for human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 ...cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye...Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090... Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID:...: Viral Vectors As part of our Viral Service , Addgene is producing ready-to-use viral preparations from...preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in...

... RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled... click on a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by ...of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. Special...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer...

... information, please visit the Addgene SAM library page . Targeted gene activation using SAM There are...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid... and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV...-Cre recombinase and sgRNAs targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains...

... ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication... Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses a 3xFLAG-tagged...retroviral expression vector with the neomycin resistance gene 64325 3xFLAG-dCas9/p-bacteria Expresses 3xFLAG-dCas9...Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573... Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520. doi: 10.1111/gtc.12492... ribonucleoproteins. Fujita T, Yuno M, Fujii H. Genes Cells. 2016 Apr;21(4):370-7. doi: 10.1111/gtc.12341...MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. Fujita T, Kitaura F, Fujii H...Lab web page . Figure 1: iChIP general scheme Figure 2: enChIP general scheme Fujii Lab Plasmids Individual...

...viral particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is ... which can be modified to encode a wide range of gene products. The main restrictions on a lentiviral ...chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan...gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions of ...pULTRA 3rd bi-cistronic expression of EGFP and the gene of interest, also can express shRNA (cloning H1-...mutagensis. The 2nd and 3rd generation lentiviral systems available at Addgene have been designed for increased...used with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253...

...-derived inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. (Link opens ...molecules/μL to generate a standard curve: One option is to use plasmid #59462 from Addgene. The values highlighted...Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 ...AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. Protocols...Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations Always run standards and samples...titer >3 x 10 13 GC/mL, use dilutions 5–8 Note: at Addgene, samples typically range from 1 x 10 12 GC/mL to...