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Showing: 61 - 80 of 1031 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...spread from this cell to synaptically connected cells (meaning, it can spread to cells that are projecting...infect a single "starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic...provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral...projecting to the starter cell). Once in the secondarily infected cells that lack the G protein, the virus ...connected cells. To further refine the targeting of G-deleted rabies to specific starter cells, the tropism...G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein. EnvA-pseudotyped...is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor. RABV...

  2. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...for one well in 24-well plate. A good example/reference is this Cell paper: http://www.cell.com/abstract... 10uM oligo stock for each well on 24-well plate, co-deliver into the cells with the Cas9/sgRNAs vector... vector, for a one well in a 24-well plate. A good reference is this paper in Cell . Click here for a ...find both single-allelic and bi-allelic cells. Single-allelic cells usually make up the majority in culture...specificity. A good reference is this Cell paper: http://www.cell.com/abstract/S0092-8674(13)01015-5 As for...allows you to sort for GFP-positive cells and to enrich for those cells that were positively transfected....CRISPR is used to engineer a locus, isolate single cell colonies, then genotype them, it is possible to ...

  3. Brain Armamentarium

    Type
    Collection
    ...channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213940-PHPeB ...expressing dTomato driven by PV+ basket cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213937-PHPeB ...channelrhodopsin-2 driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213936-PHPeB ...expressing dTomato driven by chandelier cell-targeting enhancer Gordon Fishell Viviana Gradinaru 213915-PHPeB ...Collection The Armamentarium for Precision Brain Cell Access is developing a collection of molecular genetic... reagents to gain access to many different brain cell types. The expanded collection of Armamentarium ...10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2...Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell 213855...Control Fishell 213936 pAAV_BiPVe4_dTomato_nlsdTomato BiPVe4 dTomato/nlsdTomato Control Fishell 213940 ...Control Fishell 213944 pAAV_BiSSTe4_dTomato_nlsdTomato BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics...Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3 Activator ChR2 Fishell 213937 pAAV_BiPVe4... Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 Activator ChR2 Fishell 213945 pAAV_BiSSTe4...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...OPTN His CMV ALS Jon Ashwell 23051 pDEST26-hOPTN-D474N OPTN His CMV ALS Jon Ashwell 23052 pDEST15-hOPTN...hOPTN OPTN GST T7 ALS Jon Ashwell 23053 pDEST17-hOPTN OPTN His T7 ALS Jon Ashwell 23054 pDEST15-hOPTN-D474N...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander...Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9. Cell . 167(7)...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...orthogonality, and multiplexibility in various species, as well as the development of new applications. The first...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...Current libraries are available for gene knockout, as well as transcriptional activation or repression. Combined...

  7. Rett Syndrome

    Type
    Collection
    ...information Mouse and Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository...Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells (iPSCs) are a powerful system...opens in a new window) LUHMES cell line , an immortalized pre-neuronal cell line that can be differentiated... RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines ...Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link ...X inactivation results in approximately half of cells expressing wild-type MECP2 and half expressing mutant...treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice for...

  8. Cre-lox system

    Type
    Collection
    ...mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft...Mammalian Heller 112615 pEHC Emerald and Cre-ERT2 with MCS for inserting promoter none Mammalian Heller 112616...promoter none Mammalian Heller 112618 pCAG-VHC Venus, Cre-ERT2 CAG Mammalian Heller 112619 pCAG-EHC Emerald...Cre-ERT2 CAG Mammalian Heller 112620 pCAG-mTHC TFP, Cre-ERT2 CAG Mammalian Heller 112621 pCAG-tdTHC tdTomato...allowing for direct comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool...promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is ...promoters. Expression of both N and CCre in the same cell type allows for recombination of LoxP flanked DNA...

  9. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ... mammalian cells 48880 3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD...mammalian cells 49536 3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9...Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. Fujita T, Kitaura F...enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-IG Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...3xFLAG-dCas9/pMXs-I2 Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions...

  10. Lentiviral Prep Service

    Type
    Collection
    ...carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes...barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses...carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes...carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes...knockout pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular systems typically relies on genetic... and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin...Shendure Lab Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain - Alex...Mumm Lab. NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of ...below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation - Jeffrey...Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan...aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live imaging tools to study the development...

  12. Viral Production

    Type
    Collection
    ... preparations are added to cells in culture. Three to five days later, cell cultures are inspected for... a new vial of early passage cells. Approximately 2 weeks post-thaw, cell culture supernatant is tested...involves transducing cells with serial dilutions of the lentiviral vector, harvesting cells several days later...see maps section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre... (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445...Production Lentiviral preparations are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging...packaging system developed by the Trono lab . Cell culture medium (OptiPro SFM) containing lentivirus is ...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...effectors to visualize subcellular positioning of target DNA sequences in living cells (TALE-mediated Genome...serial ligation. Validated in zebrafish somatic cells. Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate...ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised...pC-GoldyTALEN Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative..., both in microinjected embryos and transfected cells. TALEN expression is driven by the strong CAG promoter...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed for use... the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...

  14. Neurodegeneration Research Collection

    Type
    Collection
    ...protein-protein interactions and nerve cell function within the cell. Parkinson's Disease Parkinson’s disease...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of ...with the disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%)...that causes the progressive degeneration of nerve cells in the brain. Symptoms include changes in behavior...emotion, uncontrolled movements, memory deficits, as well as difficulty speaking and swallowing. HD is caused...FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere et al. Mol Neurodegener. 2023 Aug 8...system, peripheral nervous system, vesicular brain cells and more. New and Noteworthy: AAV.rTH.PI.Cre.SV40...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB...following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System The Förstemann... Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has produced...tagged, human, induced pluripotent stem cell (hiPSC) lines. These cell lines were created using CRISPR and...methods. Several different tagging techniques, as well as the plasmids and protocols needed for using them...tagging transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use...guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms for the...

  16. Viral Vectors

    Type
    Collection
    ...hard-to-transfect mammalian cells. In addition, "viral pseudotyping" can be used to target specific cells or tissues. ...genetic material into a cell, and even deliver this genetic material to specific cells in an organism. Figure...Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell Genome Integration Immune Response Lentivirus...because of their ability to transduce non-dividing cells. These viruses enable long-term stable gene expression...ability to preferentially infect and kill cancer cells. Adenoviruses have high transduction efficiency ... though this infectivity is limited to dividing cells. Retroviruses integrate permanently into the host...for the delivery of this genetic information to cells. Adapting plasmid DNA for use with virus-mediated...

  17. COVID-19 Resources

    Type
    Collection
    ... of the viral spike (S) protein to cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV...SARS-CoV-2 . TMPRSS2 - Hoffmann M, et al, 2020, Cell SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is...key step is the priming of the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed...Human Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV...2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor ...2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease...increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in beating of airway cilia...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep... combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...

  19. Retrograde AAV viral preps

    Type
    Collection
    ...-GFP-Fishell-1 Dlx GFP Control Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Fishell 83894...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hDlx Inhibitor DREADD Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hDlx Activator...83894 pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Control Fishell 104055 pAAV-CAG-eYFP CAG EYFP Control...83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 mDIx Activator Optogenetics Fishell 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2... of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164 pAAV-nEF-Con/Fon/Von eYFP...Cre-dependent Calcium sensor Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of ...of the mDlx enhancer element Calcium sensor Fishell 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,... Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new window) Mingote S, Amsellem A, Kempf ... powerful molecular reagents for biology have propelled our understanding of neural circuit functionality...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...
Showing: 61 - 80 of 1031 results