Skip to main content
Addgene
Showing: 61 - 80 of 164 results

  1. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Used for neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA...ADH1 General expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length...

  4. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...value cut off. This method entails arranging all calculated p values from smallest to largest and ranking...ranking them (1 being the smallest value). It calculates the cut off for significance by finding the largest...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...

  7. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...

  8. AAV Viral Preps

    Type
    Collection
    ...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...

  10. Deck the Lab 2022!

    Type
    Blog Post
    ... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...

  12. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...

  15. Antibody Production

    Type
    Collection
    ...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  16. Editor's Choice, September 2016

    Type
    Blog Post
    ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...

  19. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...
Showing: 61 - 80 of 164 results