Skip to main content
Addgene
Showing: 151 - 164 of 164 results

  1. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Addgene. The values highlighted below in red were calculated using this plasmid, but will change if you use...use a different plasmid. Sample Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...base pairs for each varies, it is difficult to calculate this based on DNA concentration alone. One method...

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...trendline for the standards. Google Sheets can calculate this for you as follows: In the Chart Editor ,...based on your sample’s HC + LC area. Example calculation: If the sample HC + LC Area is 18179.442 and ...

  4. DNA Quantification

    Type
    Protocol
    ...Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....

  8. Lentivirus Production

    Type
    Protocol
    ... Da Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect...

  11. Western Blot

    Type
    Protocol
    ... 37 °C . Determine the absorbance at 590 nm . Calculate the average absorbance of the duplicate samples...

  13. AAV ddPCR Titration

    Type
    Protocol
    ... a CSV file and use the following equation to calculate the titer for each sample: $$T = {R*C*D*1000\over...
Showing: 151 - 164 of 164 results