...cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated a series of plasmids that...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

...single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and...genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ...Max Planck Society. Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the ...

...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...Thermus aquaticus bacterium – was discovered at Octopus Hot Spring at YNP, and since then, many researchers...

...breaks (DSBs) or donor DNA templates.  Figure 1: An overview of prime editing. Created with BioRender.com...demonstrated ~20-50% efficiency in HEK293T cells with 1-10% indels. Since then, further innovations have continued...Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...

... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to.... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...

...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...Bioinformatics from Boston University, and did postdoctoral work at Harvard Medical School and the University...

...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...candidates for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went...

...regarding what makes this group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors...

...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

...advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize every opportunity prior...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...Guerin and Susanna Stroik. Didem Goz Ayturk is a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal...

...Bachelor's degree Generally a short-term position (1-2 years) Often a gap-year position taken to transition...teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific...scientific and professional training after earning a doctorate Considered a "stepping stone" position to become...

...basis of many molecular biology tools! Figure 1: Common functions of natural plasmids. Created with...January 2017 and was updated by Emily P. Bentley in October 2024. References and Resources References Demanèche...

.... Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research...working group (National Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95...

...supervisor, even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every ...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

...avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody... Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel... µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4...Warm reagents to room temperature. Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute...stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS...microfuge tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into 450 µL PBS in a microfuge tube and... a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10 twice for a total of three...

...7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose. 1 mg/mL polyethylenimine (PEI) ...Calculate the amount of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid ... determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / 24,961 bp...deionized water + 100 mL of 1 M Tris HCl pH 8.5 + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride...high glucose, Corning 10-013-CV DMEM, low glucose (1 g/L) glucose, sodium pyruvate, Corning 10-014-CV (... sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone SH30237.01 (optional) L-alanyl-L-glutamine...cations can affect the attachment of adherent cells) 1 mg/mL Polyethylenimine (PEI) 25 kDa MW Pro-Tip Other...

...collaborations, keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create...

...email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault...Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the...

...halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017). How to get an opsin into...then moved to France where she worked as a post-doctoral fellow on developmental biology at the École Normale...