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  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

  7. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...

  8. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...enough, but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ... knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents an attractive...! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS...

  19. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...
Showing: 761 - 780 of 986 results