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Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Learn how electronic lab notebooks can make your lab life a little easier and find tips to choose the best the electronic lab notebook for your needs.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Find 10 tips on how to give the perfect science talk

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

Introduction to immunofluorescence and how you can use it to illuminate your research.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Looking for a way to study integrins? IPI's first deposit, a toolset comprised of anti-integrin antibodies, is now available in Addgene's repository!

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.

We describe the different classes of optogenetic tools available and what levels of control they give you in your experimental setup.